Preparation and characterization of DNA films using oleylamine modified Au surfaces

被引：35

作者：

Borges, Joao ^{[1
]}

Ribeiro, Jose A. ^{[1
]}

Pereira, Elisa M. ^{[1
]}

Carreira, Catia A. ^{[1
]}

Pereira, Carlos M. ^{[1
]}

Silva, Fernando ^{[1
]}

机构：

[1] Univ Porto, Fac Ciencias, Dept Quim, Ctr Invest Quim CIQ UP,L4, P-4169007 Oporto, Portugal

来源：

JOURNAL OF COLLOID AND INTERFACE SCIENCE | 2011年 / 358卷 / 02期

关键词：

Electrostatic adsorption; Self-assembly; Thin films; OLA; dsDNA; QCM-D; SELF-ASSEMBLED MONOLAYERS; QUARTZ-CRYSTAL MICROBALANCE; MULTILAYERED POLYELECTROLYTE FILMS; DOPAMINE-INDUCED APOPTOSIS; SILVER NANOPARTICLES; GENE DELIVERY; GOLD; SPECTROSCOPY; ADSORPTION; KINETICS;

D O I：

10.1016/j.jcis.2011.03.039

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Thin films composed of oleylamine (OLA) and double-stranded deoxyribonucleic acid (dsDNA from Salmon testes) have been successfully constructed on polycrystalline Au surfaces using the electrostatic adsorption and self-assembly (SA) technique. The formation of the Au/OLA/dsDNA films was followed step-by-step by Quartz Crystal Microbalance with energy dissipation (QCM-D), Atomic Force Microscopy (AFM), and electrochemical techniques such as Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS). The use of these techniques allowed the characterization and the follow up of the successful construction of the OLA/dsDNA composite film. The main advantages of the proposed methodology are the simplicity of the modification procedure, the stability of the dsDNA self-assembled film, and the potential employment of the dsDNA modified gold electrodes to study the interactions of DNA with target molecules. Copyright (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：626 / 634

页数：9

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