Structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O60

被引：3
|
作者：
Ovchinnikova, Olga G. [1 ]
Kocharova, Nina A. [1 ]
Parkhomchuk, Angelina A. [1 ]
Bialczak-Kokot, Magdalena [2 ]
Shashkov, Alexander S. [1 ]
Knirel, Yuriy A. [1 ]
Rozalski, Antoni [2 ]
机构：
[1] Russian Acad Sci, ND Zelinsky Inst Organ Chem, Moscow 119991, Russia
[2] Univ Lodz, Dept Immunobiol Bacteria, Inst Microbiol Biotechnol & Immunol, PL-90237 Lodz, Poland
来源：
CARBOHYDRATE RESEARCH | 2011年 / 346卷 / 02期
关键词：
Providencia alcalifaciens; O-Antigen; Lipopolysaccharide; Bacterial polysaccharide structure; PROTEUS;
D O I：
10.1016/j.carres.2010.11.016
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
An O-polysaccharide (O-antigen) was isolated by mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O60 and studied by sugar and methylation analyses as well as (1)H and (13)C NMR spectroscopy, including 2D ROESY and (1)H, (13)C HMBC experiments in D(2)O and a ROESY experiment in a 9:1 H(2)O-D(2)O mixture to reveal correlations for NH protons. It was found that the polysaccharide is built up of linear pentasaccharide repeating units containing an amide of o-glucuronic acid with L-serine and has the following structure: -> 4)-beta-D-Glcp-(1 -> 3)-alpha-D-Galp-(1 -> 4)-beta-D-GalpNAc-(1 -> 4)-beta-D-GlepA-(1 -> 3)-beta-D-GalpNAc-(1 -> L-Ser-(2-6) The O-antigen studied is structurally and serologically closely related to the O-antigen of Proteus vulgaris O44. (c) 2010 Elsevier Ltd. All rights reserved.
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收藏
页码：377 / 380
页数：4
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