Injectable Methylcellulose and Hyaluronic Acid Hydrogel Containing Silver Nanoparticles for Their Effective Anti-microbial and Wound Healing Activity After Fracture Surgery

Currently, the synthesis of silver nanoparticles (AgNPs) was utilized for various life-saving biomedical applications. Herein, we report the synthesis and characterization of methylcellulose and hyaluronic acid (MC-HA) hydrogels containing AgNPs dressing materials were investigated for their wound healing efficiency to treat surgical excision wounds and the findings were studied and discussed. The effect of the ratio of AgNPs and MC-HA hydrogels on the quick gelation time maximum at 7.8 +/- 0.39 min, in vitro degradation (81.6 +/- 4.0%), and equilibrium swelling (67 +/- 3.35) of MC-HA/AgNPs hydrogels was examined. The prepared MC-HA hydrogels containing AgNPs were studied using various characterization techniques such as UV-Visible spectroscopy, Scanning electron microscopy (SEM), Transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, and X-ray diffraction (XRD). The HRTEM average diameter of the synthesized MC-HA/AgNPs was measured to be 35 nm. The MC-HA/AgNPs showed excellent antimicrobial activity against Staphylococcus aureus and Escherichia coli, respectively. Additionally, the superior wound recovering potential of AgNPs with MC-HA hydrogels compared to conventional formulations was illustrated in vitro in animals utilizing visual observations and histological study. The MC-HA/AgNPs hydrogels showed excellent antimicrobial activity and burn wound healing. Therefore, these MC-HA hydrogels containing AgNPs have great potential medicinal applications in nursing care in children after surgery. Graphic Abstract