Changes in non-22-kilodalton (kDa) isoforms of growth hormone (GH) after administration of 22-kDa recombinant human GH in trained adult males

被引:64
|
作者
Wallace, JD [1 ]
Cuneo, RC
Bidlingmaier, M
Lundberg, PA
Carlsson, L
Boguszewski, CL
Hay, J
Boroujerdi, M
Cittadini, A
Dall, R
Rosén, T
Strasburger, CJ
机构
[1] Univ Queensland, Princess Alexandra Hosp, Dept Med, Metab Res Unit, Brisbane, Qld 4102, Australia
[2] Univ Queensland, Princess Alexandra Hosp, Dept Social & Preventat Med, Stat Sect, Brisbane, Qld 4102, Australia
[3] Innenstadt Univ Hosp, Dept Med, Neuroendocrine Unit, D-80336 Munich, Germany
[4] Univ Fed Parana, Hosp Clin, Sect Endocrinol & Metabol, BR-80060240 Curitiba, Parana, Brazil
[5] Gothenburg Univ, Sahlgrens Hosp, Res Ctr Endocrinol & Metab, S-41345 Gothenburg, Sweden
[6] St Thomas Hosp, Dept Endocrinol, London SE1 7EH, England
[7] Univ Naples Federico II, Dept Internal Med & Cardiovasc Sci, I-80131 Naples, Italy
[8] Aarhus Univ Hosp, Dept Med Endocrinol & Diabet M, DK-8000 Aarhus, Denmark
来源
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM | 2001年 / 86卷 / 04期
关键词
D O I
10.1210/jc.86.4.1731
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
GH is being used by elite athletes to enhance sporting performance. To examine the hypothesis that exogenous 22-kDa recombinant human GH (rhGH) administration could be detected through suppression of non-22-kDa isoforms of GH, we studied seventeen aerobically trained males (age, 26.9 +/- 1.5 yr) randomized to rhGH or placebo treatment (0.15 IU/kg/day for 1 week). Subjects were studied at rest and in response to exercise (cycle-ergometry at 65% of maximal work capacity for 20 min). Serum was assayed for total GH (Pharmacia IRMA and pituitary GH), 22-kDa GH (2 different 2-site monoclonal immunoassays), non-22-kDa GH (22-kDa GH-exclusion assay), 20-kDa GH, and immunofunctional GH. In the study, 3 h after the last dose of rhGH, total and 22-kDa GH concentrations were elevated, reflecting exogenous 22-kDa GH. Non-22-kDa and 20-kDa GH levels were suppressed. Regression of non-22-kDa or 20-kDa GH against total or 22-kDa GH produced clear separation of treatment groups. In identical exercise studies repeated between 24 and 96 h after cessation of treatment, the magnitude of the responses of all GH isoforms was suppressed (P < 0.01), but the relative proportions were similar to those before treatment. We conclude: 1) supraphysiological doses of rhGH in trained adult males suppressed exercise-stimulated endogenous circulating isoforms of GH for up to 4 days; 2) the dearest separation of treatment groups required the simultaneous presence of high exogenous 22-kDa GH and suppressed 20-kDa or non-22-kDa GH concentrations; and 3) these methods may prove useful in detecting rhGH abuse in athletes.
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页码:1731 / 1737
页数:7
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