Establishment and characterization of GCSR1, a multi-drug resistant signet ring cell gastric cancer cell line

被引：7

作者：

Xu, Xin ^{[1
]}

Qian, Li-Juan ^{[2
]}

Su, Xing-Yun ^{[1
]}

He, Kui-Feng ^{[1
]}

Jin, Ke-Tao ^{[1
]}

Gu, Lin-Hui ^{[2
]}

Feng, Jian-Guo ^{[2
]}

Li, Guang-Liang ^{[1
]}

Zhou, Quan ^{[1
]}

Xu, Zhen-Zhen ^{[1
]}

Wang, Hao-Hao ^{[1
]}

Zhang, Jing ^{[1
]}

Cao, Jiang ^{[3
]}

Teng, Li-Song ^{[1
]}

机构：

[1] Zhejiang Univ, Affiliated Hosp 1, Dept Surg Oncol, Sch Med, Hangzhou 310003, Zhejiang, Peoples R China

[2] Zhejiang Prov Canc Hosp, Zhejiang Canc Ctr, Zhejiang Canc Res Inst, Hangzhou 310022, Zhejiang, Peoples R China

[3] Zhejiang Univ, Affiliated Hosp 2, Clin Res Ctr, Sch Med, Hangzhou 310009, Zhejiang, Peoples R China

来源：

INTERNATIONAL JOURNAL OF ONCOLOGY | 2015年 / 46卷 / 06期

基金：

中国国家自然科学基金;

关键词：

cell line; drug resistance; establishment; gastric cancer; stem cell; CD44; CD133; STEM-CELLS; CARCINOMA; ADENOCARCINOMA; IDENTIFICATION; AMPLIFICATION; SURGERY; MARKER; CD44;

D O I：

10.3892/ijo.2015.2966

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Signet ring cell gastric cancer (SRCGC) has very poor prognosis worldwide, and studying its molecular characteristics is urgent for improving the outcome. However, few well-characterized SRCGC cell lines are available for research. Therefore, we established a novel cell line GCSR1, from a Chinese male SRCGC patient. Cell morphology of GCSR1 in culture, maintained in vitro for over 90 passages, is similar to the cells from the patient. GCSR1 cells proliferated in vitro with a doubling time of 67.65 h. Karyotyping showed they were aneuploid. Missense mutation occurred in codon 193 of P53 and deletion occurred in exons 1 and 3 of P16. Results of CCK8 assay revealed that GCSR1 was more resistant to 5-fluorouracil (5-FU) and mitomycin (MMC) than other gastric cancer cell lines. Stem cell marker assay by flow cytometry showed that GCSR1 had high proportion of CD44(+) and/or CD133(+) cells. It formed colonies easily in soft agar and generated xenograft tumors in nude mice. In conclusion, GCSR1 is a well-established, well-characterized multi-drug resistant cell line with abundant cancer stem cells.

引用

页码：2479 / 2487

页数：9