Three-dimensional appearance of Langerhans cells in human gingival epithelium as revealed by confocal laser scanning microscopy

被引:11
作者
Ito, H
Takekoshi, T
Miyauchi, M
Ogawa, I
Takata, T
Nikai, H
Takemoto, K
机构
[1] Dept Oral Pathol, Minami Ku, Hiroshima 7348553, Japan
[2] Hiroshima Univ, Sch Dent, Dept Oral Pathol, Minami Ku, Hiroshima 7348553, Japan
[3] Hiroshima Univ, Dent Hosp, Clin Lab, Minami Ku, Hiroshima 7348553, Japan
关键词
Langerhans cells; human gingiva; confocal laser scanning microscopy; three-dimensional reconstruction; immunofluorescence;
D O I
10.1016/S0003-9969(98)00066-1
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Detailed three-dimensional morphological information of the cell without distortion due to thin sectioning can be obtained from thick sections using a confocal laser scanning microscope. Here that microscope was used to evaluate human gingival Langerhans cells stained with anti-CD1a antibody. Optical sectioning and reconstruction by laser scanning microscopy revealed not only three-dimensional aspects of Langerhans cells but also spatial information on the distribution of their dendritic processes towards the gingival surface. The orientated configuration of those processes may reflect the reaction of the gingival Langerhans cell to the surrounding moist environment containing antigenic stimuli originating in saliva. (C) 1998 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:741 / 744
页数:4
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