Infarct stabilization and cardiac repair with a VEGF-conjugated, injectable hydrogel

Injectable scaffolds made of biodegradable biomaterials can stabilize a myocardial infarct and promote cardiac repair Here we describe the synthesis of a new temperature-sensitive aliphatic polyester hydrogel (HG) conjugated with vascular endothelial growth factor (VEGF) and evaluate its effects on cardiac recovery after a myocardial infarction (MI) Seven days after coronary ligation in rats PBS HG or HG mixed or conjugated with VEGF (HG + VEGF or HG-VEGF respectively) was injected around the infarct (n = 8-11/group) Function was evaluated by echocardiography at multiple time points Pressure volume measurements were taken and infarct morphometry and blood vessel density were assessed at 35 days after injection HG-VEGF provided localized sustained VEGF function Compared with outcomes in the PBS group fractional shortening ventricular volumes preload recruitable stroke work and end-systolic elastance were all preserved (p < 0 05) in the HG and HG + VEGF groups and further preserved in the HG-VEGF group Conjugated VEGF also produced the highest blood vessel density (p < 0 05) The infarct thinned and dilated after PBS injection but was smaller and thicker in hearts treated with HG (p < 0 05) Our temperature-sensitive HG attenuated adverse cardiac remodeling and improved ventricular function when injected after an MI VEGF delivery enhanced these effects when the VEGF was conjugated to the HG (C) 2010 Elsevier Ltd All rights reserved