Two methods for glass surface modification and their application in protein immobilization

被引：103

作者：

Qin, Ming

Hou, Sen

Wang, LiKai

Feng, XiZeng ^{[1
]}

Wang, Rui

Yang, YanLian

Wang, Chen

Yu, Lei

Shao, Bin

Qiao, MingQiang

机构：

[1] Nankai Univ, Coll Life Sci, Minist Educ, Key Lab Bioact Mat, Tianjin 300071, Peoples R China

[2] Natl Ctr Nanosci & Technol, Beijing 100080, Peoples R China

[3] Nankai Univ, Coll Life Sci, Inst Mol Biol, Tianjin 300071, Peoples R China

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2007年 / 60卷 / 02期

基金：

中国国家自然科学基金;

关键词：

hydrophobin; HFBI; protein adsorption; glass; surface modification;

D O I：

10.1016/j.colsurfb.2007.06.018

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Protein immobilization is a crucial step in protein chip, biosensor, etc. Here, two methods to immobilize proteins on glass surface were analyzed, one is silanization method using 3-aminopropyltriethoxysilane (APTES), and the other is hydrophobin HFBI coating. The modified glass surfaces were characterized with X-ray photoelectron spectroscopy (XPS), water contact angle measurement (WCA) and immunoassay. The results of XPS and WCA illustrated that the surface property of glass can be changed by both the two methods. The following immunoassay using microcontact printing (mu CP) verified that both methods could help protein immobilization effectively on glass slides. Compared with the amine treatment, it is concluded that hydrophobin self-assemblies is a simple and generic way for protein immobilization on glass slides, which has potential application in protein chips and biosensors. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：243 / 249

页数：7

共 59 条

[1] Interaction and comparison of a class I hydrophobin from Schizophyllum commune and class II hydrophobins from Trichoderma reesei [J].

Askolin, S ;

Linder, M ;

Scholtmeijer, K ;

Tenkanen, M ;

Penttilä, M ;

de Vocht, ML ;

Wösten, HAB .

BIOMACROMOLECULES, 2006, 7 (04) :1295-1301

[2]

Benters R, 2001, CHEMBIOCHEM, V2, P686, DOI 10.1002/1439-7633(20010903)2:9<686::AID-CBIC686>3.0.CO

[3]

2-S

[4]

Bernard A, 2000, ADV MATER, V12, P1067, DOI 10.1002/1521-4095(200007)12:14<1067::AID-ADMA1067>3.0.CO

[5]

2-M

[6] Printing patterns of proteins [J].

Bernard, A ;

Delamarche, E ;

Schmid, H ;

Michel, B ;

Bosshard, HR ;

Biebuyck, H .

LANGMUIR, 1998, 14 (09) :2225-2229

[7] EFFECT OF A VIRUS ON ACCUMULATION OF A TISSUE-SPECIFIC CELL-SURFACE PROTEIN OF THE FUNGUS CRYPHONECTRIA-(ENDOTHIA)-PARASITICA [J].

CARPENTER, CE ;

MUELLER, RJ ;

KAZMIERCZAK, P ;

ZHANG, L ;

VILLALON, DK ;

VANALFEN, NK .

MOLECULAR PLANT-MICROBE INTERACTIONS, 1992, 5 (01) :55-61

[8]

CATHERINE MH, 2001, ANAL CHEM, V73, P2476

[9] Making and reading microarrays [J].

Cheung, VG ;

Morley, M ;

Aguilar, F ;

Massimi, A ;

Kucherlapati, R ;

Childs, G .

NATURE GENETICS, 1999, 21 (Suppl 1) :15-19

[10] A novel two-step extraction method with detergent/polymer systems for primary recovery of the fusion protein endoglucanase I-hydrophobin I [J].

Collén, A ;

Persson, J ;

Linder, M ;

Nakari-Setälä, T ;

Penttilä, M ;

Tjerneld, F ;

Sivars, U .

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2002, 1569 (1-3) :139-150

← 1 2 3 4 5 6 →