Fluorescence Quenching and Enhancement of DNA-Templated Silver Nanocluster by DNA Hybridization

被引：3

作者：

Lee, Chunghyun ^{[1
]}

Gang, Jongback ^{[1
]}

机构：

[1] Gachon Univ, Dept Nano Chem, Sungnam 461701, Kyunggido, South Korea

来源：

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | 2017年 / 17卷 / 11期

关键词：

DNA-Templated Silver Nanocluster; Fluorescence Quenching; Electron Transfer; Mismatched Base; LABEL-FREE; ENDONUCLEASE ACTIVITY; BASE-PAIR; PROBE; DEACTIVATION; FLUOROPHORES; SELECTIVITY; INHIBITION; SEQUENCE;

D O I：

10.1166/jnn.2017.15077

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Oligonucleotide-templated Ag nanoclusters (DNA-AgNCs) has been widely used to detect biomolecules of interest due to the useful properties. To understand fluorescence quenching and enhancement of DNA-AgNCs by cDNA, this study used cDNA containing several mismatched bases (cDNA-1) and the full-length cDNA with a mismatched base (f-cDNA-1). We showed that DNA-AgNCs decreased fluorescence intensities to different degrees by hybridization of cDNA and cDNA-1. However, the fluorescence intensities of DNA-AgNCs in the presence of f-cDNA and f-cDNA-1 increased by 153% and 491%, respectively, compared to that of DNA-AgNCs, respectively.

引用

页码：7961 / 7965

页数：5

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