Genomic Analysis of Carbapenemase-Producing Extensively Drug-Resistant Klebsiella pneumoniae Isolates Reveals the Horizontal Spread of p18-43_01 Plasmid Encoding blaNDM-1 in South Africa

被引：25

作者：

Ramsamy, Yogandree ^{[1
,2
,3
]}

Mlisana, Koleka P. ^{[2
,3
]}

Allam, Mushal ^{[4
]}

Amoako, Daniel G. ^{[1
]}

Abia, Akebe L. K. ^{[1
]}

Ismail, Arshad ^{[4
]}

Singh, Ravesh ^{[2
,3
]}

Kisten, Theroshnie ^{[5
,6
]}

Han, Khine Swe Swe ^{[2
,3
]}

Muckart, David J. Jackson ^{[7
,8
]}

Hardcastle, Timothy ^{[7
,8
]}

Suleman, Moosa ^{[9
]}

Essack, Sabiha Y. ^{[1
]}

机构：

[1] Univ KwaZulu Natal, Coll Hlth Sci, Antimicrobial Res Unit, ZA-4000 Durban, South Africa

[2] Natl Hlth Lab Serv, Med Microbiol, ZA-4000 Durban, South Africa

[3] Univ KwaZulu Natal, Coll Hlth Sci, Med Microbiol, ZA-4000 Durban, South Africa

[4] Natl Hlth Lab Serv, Natl Inst Communicable Dis, Sequencing Core Facil, ZA-2131 Johannesburg, South Africa

[5] Univ KwaZulu Natal, Coll Hlth Sci, Sch Clin Med, Discipline Anaesthet & Crit Care, ZA-4000 Durban, South Africa

[6] Univ KwaZulu Natal, Inkosi Albert Luthuli Cent Hosp, Coll Hlth Sci, Dept Crit Care, ZA-4000 Durban, South Africa

[7] Univ KwaZulu Natal, Inkosi Albert Luthuli Cent Hosp, Coll Hlth Sci, Dept Surg, ZA-4000 Durban, South Africa

[8] Univ KwaZulu Natal, Inkosi Albert Luthuli Cent Hosp, Coll Hlth Sci, Trauma Unit, ZA-4000 Durban, South Africa

[9] Ahmed Al Kadi Private Hosp, ZA-4000 Durban, South Africa

来源：

MICROORGANISMS | 2020年 / 8卷 / 01期

基金：

新加坡国家研究基金会; 英国医学研究理事会;

关键词：

genomics; carbapenemase; Klebsiella pneumoniae; extensively drug-resistant; mobile genetic elements; epidemiology; phylogenomic; South Africa; ANTIMICROBIAL RESISTANCE; BETA-LACTAMASE; ENTEROBACTERIACEAE; PREVALENCE; SUSCEPTIBILITY; EPIDEMIOLOGY; EVOLUTION; BACTERIAL; CARRIAGE; IMPACT;

D O I：

10.3390/microorganisms8010137

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Whole-genome sequence (WGS) analyses were employed to investigate the genomic epidemiology of extensively drug-resistant Klebsiella pneumoniae strains, focusing on the carbapenem resistance-encoding determinants, mobile genetic support, clonal and epidemiological relationships. A total of ten isolates were obtained from patients admitted to the intensive care unit (ICU) in a public hospital in South Africa. Five isolates were from rectal swabs of colonized patients and five from blood cultures of patients with invasive carbapenem-resistant infections. Following microbial identification and antibiotic susceptibility tests, the isolates were subjected to WGS on the Illumina MiSeq platform. All the isolates showed genotypic resistance to tested beta-lactams (NDM-1, OXA-1, CTX-M-15, TEM-1B, SHV-1) and other antibiotics. All but one isolate belonged to the ST152 with a novel sequence type, ST3136, differing by a single-locus variant. The isolates had the same plasmid multilocus sequence type (IncF[K12:A-:B36]) and capsular serotype (KL149), supporting the epidemiological linkage between the clones. Resistance to carbapenems in the 10 isolates was conferred by the bla(NDM-1) mediated by the acquisition of multi-replicon [ColRNAI, IncFIB(pB171), Col440I, IncFII, IncFIB(K) and IncFII(Yp)] p18-43_01 plasmid. These findings suggest that the acquisition of bla(NDM-1)-bearing plasmid structure (p18-43_01), horizontal transfer and clonal dissemination facilitate the spread of carbapenemases in South Africa. This emphasizes the importance of targeted infection control measures to prevent dissemination.

引用

页数：16

共 1 条

[1] Pathogenomic Analysis of a Novel Extensively Drug-Resistant Citrobacter freundii Isolate Carrying a blaNDM-1 Carbapenemase in South Africa
Ramsamy, Yogandree
Mlisana, Koleka P.
Amoako, Daniel G.
Allam, Mushal
Ismail, Arshad
Singh, Ravesh
Abia, Akebe Luther King
Essack, Sabiha Y.
PATHOGENS, 2020, 9 (02):

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