Carbon Monoxide has Antioxidative Properties in the Liver Involving p38 MAP Kinase Pathway in a Murine Model of Systemic Inflammation

Objective. Reactive oxygen species (ROS) are important in the hepatocellular injury process during a systemic inflammation We examined the role of carbon monoxide (CO) on the hepatic generation of ROS with in-vivo and in-vitro models of systemic inflammation. Methods. Using a murine model of bilateral hindlimb ischemia-reperfusion (I/R) we examined the effect of CO treatment on hepatic ROS formation, oxidative status, and cell injury Cultured HUVEC were used to investigate intracellular pathways Results: CO treatment reduced hepatic lipid peroxidation, re-established total hepatic glutathione and glutathione disulfide (GSH/GSSG) levels and reduced hepatocellular injury Inhibition of heme oxygenase (HO) during treatment with CO during hindlimb I/R failed to alter the antioxidant qualities provided by CO The production of ROS after tumor necrosis factor-alpha (TNF-alpha) stimulation in HUVEC was diminished alter exposure to CO Treatment with CO dining HO inhibition reduced both ROS formation and cell injury Inhibiting the p38 MAPK (mitogen-activated protein kinase) pathway with pyridinyl imidazol (SB203580) revealed that the antioxidant potential of CO involved the activation of p38 MAPK Conclusions. CO has direct antioxidant potential independently of any HO activity during systemic inflammation The antioxidant effects afforded by CO involve the activation of the p38 MAPK pathway