Niemann-Pick C1 Heterogeneity of Bat Cells Controls Filovirus Tropism

被引：17

作者：

Takadate, Yoshihiro ^{[1
]}

Kondoh, Tatsunari ^{[1
]}

Igarashi, Manabu ^{[1
,2
]}

Maruyama, Junki ^{[1
,6
]}

Manzoor, Rashid ^{[1
]}

Ogawa, Hirohito ^{[3
,4
,7
]}

Kajihara, Masahiro ^{[1
]}

Furuyama, Wakako ^{[5
]}

Sato, Masahiro ^{[1
]}

Miyamoto, Hiroko ^{[1
]}

Yoshida, Reiko ^{[1
]}

Hill, Terence E. ^{[6
]}

Freiberg, Alexander N. ^{[6
]}

Feldmann, Heinz ^{[5
]}

Marzi, Andrea ^{[5
]}

Takada, Ayato ^{[1
,2
,4
]}

机构：

[1] Hokkaido Univ, Res Ctr Zoonosis Control, Div Global Epidemiol, Sapporo, Hokkaido 0010020, Japan

[2] Hokkaido Univ, Global Inst Collaborat Res & Educ, Global Stn Zoonosis Control, Sapporo, Hokkaido 0010020, Japan

[3] Univ Zambia, Hokudai Ctr Zoonosis Control Zambia, Sch Vet Med, Lusaka 10101, Zambia

[4] Univ Zambia, Sch Vet Med, Dept Dis Control, Lusaka 10101, Zambia

[5] NIAID, Lab Virol, Div Intramural Res, NIH,Rocky Mt Labs, Hamilton, MT 59840 USA

[6] Univ Texas Med Branch, Dept Pathol, Galveston, TX 77555 USA

[7] Okayama Univ, Dept Virol, Dent & Pharmaceut Sci, Grad Sch Med, Okayama 7008558, Japan

来源：

CELL REPORTS | 2020年 / 30卷 / 02期

关键词：

EGYPTIAN ROUSETTE BATS; EBOLA-VIRUS; FRUIT BATS; CHOLESTEROL TRANSFER; ANTIBODIES; GLYCOPROTEIN; INFECTION; PROTEIN; AEGYPTIACUS; EXPRESSION;

D O I：

10.1016/j.celrep.2019.12.042

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Fruit bats are suspected to be natural hosts of filoviruses, including Ebola virus (EBOV) and Marburg virus (MARV). Interestingly, however, previous studies suggest that these viruses have different tropisms depending on the bat species. Here, we show a molecular basis underlying the host-range restriction of filoviruses. We find that bat-derived cell lines FBKT1 and ZFBK13-76E show preferential susceptibility to EBOV and MARV, respectively, whereas the other bat cell lines tested are similarly infected with both viruses. In FBKT1 and ZFBK13-76E, unique amino acid (aa) sequences are found in the Niemann-Pick C1 (NPC1) protein, one of the cellular receptors interacting with the filovirus glycoprotein (GP). These aa residues, as well as a few aa differences between EBOV and MARV GPs, are crucial for the differential susceptibility to filoviruses. Taken together, our findings indicate that the heterogeneity of bat NPC1 orthologs is an important factor controlling filovirus species-specific host tropism.

引用

页码：308 / +

页数：17