Gram-staining characterisation of activated sludge filamentous bacteria by automated colour analysis

被引:13
作者
Pandolfi, D [1 ]
Pons, MN [1 ]
机构
[1] Ecole Natl Super Ind Chim, INPL, CNRS, Lab Sci Genie Chim, F-54001 Nancy, France
关键词
activated sludge; colour image analysis; Gram staining; population shift;
D O I
10.1007/s10529-004-5280-7
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
An automated image analysis method has been developed for the monitoring of the Gram-staining characteristics of filamentous bacteria in activated sludge. The binary method of pixel classification agreed with manual estimation (level of correlation of 0.9 for Gram-positive bacteria). Its robustness has been assessed by repeatability tests. Population shifts in terms of Gram-staining characteristics have been monitored in laboratory-scale experiments with two feeding schedules using this technique.
引用
收藏
页码:1841 / 1846
页数:6
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