Purification and characterization of a superoxide dismutase from Panax ginseng

被引：17

作者：

Li, Hong Y. ^{[1
]}

Zhao, Yu ^{[1
]}

Cao, Yang ^{[1
]}

Wang, Wei N. ^{[1
]}

Zhao, Da Q. ^{[1
]}

机构：

[1] Changchun Univ Tradit Chinese Med, Ctr New Med Res, Changchun 130117, Peoples R China

来源：

BIOMEDICAL CHROMATOGRAPHY | 2010年 / 24卷 / 11期

关键词：

Ginseng; SOD; characterization; PLANT-TISSUE CULTURE; PROTEIN; ROOT; MECHANISM;

D O I：

10.1002/bmc.1428

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A superoxide dismutase (SOD) with the molecular weight of 31,079 has been purified as a homodimer from Panax ginseng by employing neutral pH buffer extraction, ammonium sulfate precipitation, isoelectric point precipitation and ion exchange methods The enzyme's specific activity determined by an improved Marklund method was 9480 43 U/mg Metal analysis showed that the SOD contained iron with the stoichiometry of 0 9 +/- 0 3 Fe/subunit and exhibited high thermal stability (70 degrees C) over the pH range from 4 0 to 9 0 Its maximum absorption wavelength was 278 nm and it was sensitive to hydrogen peroxide, trichloromethane-ethanol and urea These results indicate that the enzyme is an iron SOD Copyright (C) 2010 John Wiley & Sons, Ltd

引用

页码：1203 / 1207

页数：5

共 50 条

[21] Purification and characterization of iron-cofactored superoxide dismutase from Enteromorpha linza
Mingsheng Lü
Ruanhong Cai
Shujun Wang
Zhaopu Liu
Yuliang Jiao
Yaowei Fang
Xiaoxin Zhang
Chinese Journal of Oceanology and Limnology, 2013, 31 : 1190 - 1195
[22] Purification, characterization, and molecular cloning of a thermostable superoxide dismutase from Thermoascus aurantiacus
E, Shijin
Guo, Fangxian
Liu, Shouan
Chen, Jing
Wang, Yanjun
Li, Duochuan
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2007, 71 (04) : 1090 - 1093
[23] Purification and characterization of iron-containing superoxide dismutase from Lentinus edodes
Park, SS
Hwang, SM
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 1999, 9 (06) : 854 - 860
[24] Purification and characterization of iron-cofactored superoxide dismutase from Enteromorpha linza
吕明生
蔡阮鸿
王淑军
刘兆普
焦豫良
房耀维
张小昕
Journal of Oceanology and Limnology, 2013, (06) : 1190 - 1195
[25] PURIFICATION OF AN ANTIULCER POLYSACCHARIDE FROM THE LEAVES OF PANAX-GINSENG
SUN, XB
MATSUMOTO, T
YAMADA, H
PLANTA MEDICA, 1992, 58 (05) : 445 - 448
[26] Microwave-Assisted Extraction, Purification, Partial Characterization, and Bioactivity of Polysaccharides from Panax ginseng
Zhao, Jing-Li
Zhang, Meiping
Zhou, Hong-Li
MOLECULES, 2019, 24 (08)
[27] SUPEROXIDE-DISMUTASE OF CRYPTOCOCCUS-NEOFORMANS - PURIFICATION AND CHARACTERIZATION
TESFASELASE, F
HAY, RJ
JOURNAL OF MEDICAL AND VETERINARY MYCOLOGY, 1995, 33 (04): : 253 - 259
[28] PURIFICATION AND CHARACTERIZATION OF ISOENZYMES OF SUPEROXIDE-DISMUTASE OF GRAIN GERM
MARMOCCHI, F
VENARDI, G
CAULINI, G
ROTILIO, G
BOLLETTINO DELLA SOCIETA ITALIANA DI BIOLOGIA SPERIMENTALE, 1974, 50 (14): : 1084 - 1090
[29] DIROFILARIA-IMMITIS SUPEROXIDE-DISMUTASE - PURIFICATION AND CHARACTERIZATION
CALLAHAN, HL
CROUCH, RK
JAMES, ER
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1991, 49 (02) : 245 - 252
[30] Purification and partial characterization of Cu/Zn superoxide dismutase from Kluyveromyces marxianus yeast
Nedeva, Trayana
Dolashka-Angelova, Pavlina
Moshtanska, Vesela
Voelter, Wolfgang
Petrova, Ventzislava
Kujumdzieva, Anna
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2009, 877 (29): : 3529 - 3536

← 1 2 3 4 5 →