Rapid and accurate direct antibiotic susceptibility testing of blood culture broths using MALDI Sepsityper combined with the BD Phoenix automated system

被引：23
作者：
Hazelton, Briony [1 ]
Thomas, Lee C. [1 ]
Olma, Thomas [1 ]
Kok, Jen [1 ,2 ,3 ]
O'Sullivan, Matthew [1 ,2 ]
Chen, Sharon C. -A. [1 ,2 ]
Iredell, Jonathan R. [1 ,2 ,3 ]
机构：
[1] Westmead Hosp, Inst Clin Pathol & Med Res, Ctr for Infect Dis & Microbiol Lab Serv, Westmead, NSW 2145, Australia
[2] Westmead Hosp, Westmead Millennium Inst, Ctr Res Excellence Crit Infect, Westmead, NSW 2145, Australia
[3] Univ Sydney, Westmead Hosp, Marie Bashir Inst Infect Dis & Biosecur, Westmead, NSW 2145, Australia
来源：
JOURNAL OF MEDICAL MICROBIOLOGY | 2014年 / 63卷
关键词：
ESCHERICHIA-COLI; IDENTIFICATION; TIME; BLA(IMP-4); BACTERIA; SEPSIS;
D O I：
10.1099/jmm.0.075580-0
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Antibiotic susceptibility testing with the BD Phoenix system on bacterial cell pellets generated from blood culture broths using the Bruker MALDI Sepsityper kit was evaluated. Seventy-six Gram-negative isolates, including 12 with defined multi-resistant phenotypes, had antibiotic susceptibility testing (AST) performed by Phoenix on the cell pellet in parallel with conventional methods. In total, 1414/1444 (97.9%) of susceptibility tests were concordant, with only 1 (0.07%) very major error. This novel method has the potential to reduce the turnaround time for AST results by up to a day for Gram-negative bacteraemias.
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收藏
页码：1590 / 1594
页数：5
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