Development of normal mice from oocytes injected with freeze-dried spermatozoa

被引：287

作者：

Wakayama, T

Yanagimachi, R ^{[1
]}

机构：

[1] Univ Hawaii, Dept Anat & Reprod Biol, Sch Med, Honolulu, HI 96822 USA

[2] Univ Tokyo, Dept Vet Anat, Fac Agr, Bunkyo Ku, Tokyo 113, Japan

来源：

NATURE BIOTECHNOLOGY | 1998年 / 16卷 / 07期

关键词：

animal husbandry; reproduction; development; fertilization;

D O I：

10.1038/nbt0798-639

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Freeze-dried mouse spermatozoa are all motionless and dead in the conventional sense. When injected into oocytes, however, their nuclei can support normal embryonic development even after three month preservation in a dried state. Although the freeze-drying protocol reported here will need further improvement, the results suggest that it may be possible to store the male genomes at room temperature.

引用

页码：639 / 641

页数：3

共 32 条

[1] OCCURRENCE AND POSSIBLE FUNCTIONAL SIGNIFICANCE OF -S-S- CROSSLINKS IN SPERM HEADS, WITH PARTICULAR REFERENCE TO EUTHERIAN MAMMALS [J].