Chromatographic method for determination of the free amino acid content of chamomile flowers

被引：15
作者：
Ma, Xiaoli [1 ]
Zhao, Dongsheng [1 ]
Li, Xinxia [1 ]
Meng, Lei [1 ]
机构：
[1] Xinjiang Med Univ, Sch Pharm, Analyt & Testing Ctr, Urumqi 830011, Xiangjiang, Peoples R China
来源：
PHARMACOGNOSY MAGAZINE | 2015年 / 11卷 / 41期
基金：
中国国家自然科学基金;
关键词：
Amino acids; 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate; Matricaria chamomilla; Matricaria recutita; reversed-phase high performance liquid chromatography; MATRICARIA-RECUTITA; EXTRACTS;
D O I：
10.4103/0973-1296.149735
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Objective: To determine the free amino acid contents of chamomile flowers using reverse-phase high-performance column chromatography preceded by pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC), and to determine the reliability of this method. Materials and Methods: Derivatization with reconstituted AQC was used to prepare the samples and standards for injection into the chromatography column. The peaks were analyzed by fluorescence detection ( excitation, 250 nm; emission, 395 nm. Results: Alanine, proline, and leucine were the most abundant amino acids, whereas tyrosine and methionine were the least abundant. The linearity of the method was found to be good with amino acid concentrations of 0.012-0.36 M. The precision was 0.05-1.36%; average recovery, 91.12-129.41%; and limit of detection, 0.006-0.058 M. Conclusion: The method is reliable for determining the free amino acid content of different types of chamomile flowers.
引用
收藏
页码：176 / 179
页数：4
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