Production of biovanillin from wheat bran

被引:41
作者
Di Gioia, Diana
Sciubba, Luigi
Setti, Leonardo
Luziatelli, Francesca
Ruzzi, Maurizio
Zanichelli, Dario
Fava, Fabio
机构
[1] Univ Bologna, Alma Mater Stn, DICASM, Fac Engn, I-40136 Bologna, Italy
[2] Univ Bologna, Dept Ind Chem & Mat Sci, I-40136 Bologna, Italy
[3] Univ Viterbo, Dept Agrobiol & Agrochem, I-01100 Viterbo, Italy
关键词
biovanillin; wheat bran; agro-industrial by-products; ferulic acid; bioconversion;
D O I
10.1016/j.enzmictec.2007.04.003
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The fine specialty chemicals production from agro-industrial by-products such as cereal bran is a topic of high interest in wheat-producing countries. In this work, the possibility of obtaining vanillin from bioconversion of ferulic acid derived from enzymatic hydrolysis of wheat bran was explored. Freely available ferulic acid was first obtained through wheat bran hydrolysis by using commercially available, technical-grade enzyme preparations. Then, resting cells of an engineered E. coli strain, JM 109(pBB 1), containing the genes responsible for conversion of ferulic acid into vanillin, were used to transform ferulic acid available in the hydrolyzed mixture into vanillin. A vanillin molar yield of 50% was obtained, but most of the vanillin produced was reduced to vanillyl alcohol. The purification of the hydrolyzate with a ionic exchange resin to remove most of the carbohydrates allowed to obtain both higher bioconversion yields (up to 70%) and lower vanillin reduction. This is the first work in which vanillin is produced from ferulic acid obtained through wheat bran hydrolysis. The results obtained demonstrate the potential application of the proposed strategy in vanillin biosynthesis from agro-industrial by-products. (c) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码:498 / 505
页数:8
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