Repeat-based Sequence Typing of Carnobacterium maltaromaticum

被引:4
|
作者
Rahman, Abdur [1 ,2 ]
El Kheir, Sara M. [1 ]
Back, Alexandre [1 ]
Mangavel, Cecile [1 ]
Revol-Junelles, Anne-Marie [1 ]
Borges, Frederic [1 ]
机构
[1] Univ Lorraine, Lab Ingn Biomol LIBio, ENSAIA, ISA, 2 Ave Foret Haye, F-406025451 Vandoeuvre Les Nancy, France
[2] Natl Univ Sci & Technol, Atta Ur Rahman Sch Appl Biosci, Dept Ind Biotechnol, H-12, Islamabad 44000, Pakistan
关键词
MULTILOCUS VARIABLE NUMBER; LISTERIA-MONOCYTOGENES; LMA; 28; PISCICOLA; INHIBITION; STRAINS;
D O I
10.1016/j.ijfoodmicro.2016.03.003
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Carnobacterium maltaromaticum is a Lactic Acid Bacterium (LAB) of technological interest for the food industry, especially the dairy as bioprotection and ripening flora. The industrial use of this LAB requires accurate and resolutive typing tools. A new typing method for C. maltaromaticum inspired from MLVA analysis and called Repeat-based Sequence Typing (RST) is described. Rather than electrophoresis analysis, our RST method is based on sequence analysis of multiple loci containing Variable-Number Tandem-Repeats (VNTRs). The method described here for C. maltaromaticum relies on the analysis of three VNTR loci, and was applied to a collection of 24 strains. For each strain, a PCR product corresponding to the amplification of each VNTR loci was sequenced. Sequence analysis allowed delineating 11, 11, and 12 alleles for loci VNTR-A, VNTR-B, and VNTR-C, respectively. Considering the allele combination exhibited by each strain allowed defining 15 genotypes, ending in a discriminatory index of 0.94. Comparison with MIST revealed that both methods were complementary for strain typing in C. maltaromaticum. (C) 2016 Elsevier B.V. All rights reserved.
引用
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页码:1 / 4
页数:4
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