Coordinate Induction of Humoral and Spike Specific T-Cell Response in a Cohort of Italian Health Care Workers Receiving BNT162b2 mRNA Vaccine

被引：47

作者：

Agrati, Chiara ^{[1
]}

Castilletti, Concetta ^{[1
]}

Goletti, Delia ^{[1
]}

Meschi, Silvia ^{[1
]}

Sacchi, Alessandra ^{[1
]}

Matusali, Giulia ^{[1
]}

Bordoni, Veronica ^{[1
]}

Petrone, Linda ^{[1
]}

Lapa, Daniele ^{[1
]}

Notari, Stefania ^{[1
]}

Vanini, Valentina ^{[1
]}

Colavita, Francesca ^{[1
]}

Aiello, Alessandra ^{[1
]}

Agresta, Alessandro ^{[1
]}

Farroni, Chiara ^{[1
]}

Grassi, Germana ^{[1
]}

Leone, Sara ^{[1
]}

Vaia, Francesco ^{[1
]}

Capobianchi, Maria Rosaria ^{[1
]}

Ippolito, Giuseppe ^{[1
]}

Puro, Vincenzo ^{[1
]}

机构：

[1] Natl Inst Infect Dis INMI L Spallanzani IRCCS, Via Portuense 292, I-00149 Rome, Italy

来源：

MICROORGANISMS | 2021年 / 9卷 / 06期

关键词：

SARS-CoV2; mRNA vaccine; health care workers; whole blood T cell assay; coordinate immunity;

D O I：

10.3390/microorganisms9061315

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Vaccination is the main public health measure to reduce SARS-CoV-2 transmission and hospitalization, and a massive worldwide scientific effort resulted in the rapid development of effective vaccines. This work aimed to define the dynamics of humoral and cell-mediated immune response in a cohort of health care workers (HCWs) who received a two-dose BNT162b2-mRNA vaccination. The serological response was evaluated by quantifying the anti-RBD and neutralizing antibodies. The cell-mediated response was performed by a whole blood test quantifying Th1 cytokines (IFN-gamma, TNF-alpha, IL-2), produced in response to spike peptides. The BNT162b2-mRNA vaccine induced both humoral and cell-mediated immune responses against spike peptides in virtually all HCWs without previous SARS-CoV-2 infection, with a moderate inverse relation with age in the anti-RBD response. Spike-specific T cells produced several Th1 cytokines (IFN-gamma, TNF-alpha, and IL-2), which correlated with the specific-serological response. Overall, our study describes the ability of the BNT162b2 mRNA vaccine to elicit a coordinated neutralizing humoral and spike-specific T cell response in HCWs. Assessing the dynamics of these parameters by an easy immune monitoring protocol can allow for the evaluation of the persistence of the vaccine response in order to define the optimal vaccination strategy.

引用

页数：7

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