HUman MicroNucleus Project:: International database comparison for results with the cytokinesis-block micronucleus assay in human lymphocytes:: I.: Effect of laboratory protocol, scoring criteria, and host factors on the frequency of micronuclei

被引：2

作者：

Bonassi, S

Fenech, M

Lando, C

Lin, YP

Ceppi, M

Chang, WP

Holland, N

Kirsch-Volders, M

Zeiger, E

Ban, SY

Barale, R

Bigatti, MP

Bolognesi, C

Jia, C

Di Giorgio, M

Ferguson, LR

Fucic, A

Lima, OG

Hrelia, P

Krishnaja, AP

Lee, TK

Migliore, L

Mikhalevich, L

Mirkova, E

Mosesso, P

Müller, WU

Odagiri, Y

Scarfi, MR

Szabova, E

Vorobtsova, I

Vral, A

Zijno, A

机构：

[1] Ist Nazl Ric Canc, Dept Environm Epidemiol, I-16132 Genoa, Italy

[2] CSIRO, Adelaide, SA, Australia

[3] Natl Yang Ming Univ, Sch Med, Inst Environm Hlth Sci, Taipei 112, Taiwan

[4] Univ Calif Berkeley, Sch Publ Hlth, Berkeley, CA 94720 USA

[5] Free Univ Brussels, Lab Cell Genet, Brussels, Belgium

[6] NIEHS, Environm Toxicol Program, Res Triangle Pk, NC 27709 USA

[7] Radiat Effects Res Fdn, Dept Radiobiol, Hiroshima, Japan

[8] Univ Pisa, Dipartimento Sci Uomo & Ambiente, Pisa, Italy

[9] Univ Turin, Dipartimento Biol Anim, I-10123 Turin, Italy

[10] Ist Nazl Ric Canc, Toxicol Evaluat Unit, I-16132 Genoa, Italy

[11] Third Mil Med Univ, Mol Toxicol Lab, Chongqing, Peoples R China

[12] Autoridad Regulatoria Nucl, Lab Dosimetria Biol, Buenos Aires, DF, Argentina

[13] Auckland Canc Soc, Res Ctr, Auckland, New Zealand

[14] Inst Med Res & Occupat Hlth, Zagreb, Croatia

[15] Ctr Protecc & Higiene Radiaciones, Havana, Cuba

[16] Univ Bologna, Dept Pharmacol, Bologna, Italy

[17] Bhabha Atom Res Ctr, Div Cell Biol, Bombay 400085, Maharashtra, India

[18] E Carolina Univ, Sch Med, Leo Jenkins Canc Ctr, Dept Radiat Oncol, Greenville, NC USA

[19] Belarus Acad Sci, Inst Cytol & Genet, Minsk, BELARUS

[20] Natl Ctr Hyg Med Ecol & Nutr, Sofia, Bulgaria

[21] Univ Tuscia, Dipartimento Agrobiol & Agrochim, I-01100 Viterbo, Italy

[22] Univ Essen Gesamthsch Klinikum, Inst Med Strahlenbiol, D-4300 Essen, Germany

[23] Yamanashi Prefectural Coll Nursing, Div Human & Hlth Sci, Kofu, Yamanashi, Japan

[24] CNR, Ist Ric Elettromagnetismo Componendi Elettron, I-80125 Naples, Italy

[25] Inst Prevent & Clin Med, Bratislava, Slovakia

[26] Cent Res Inst Roentgenol & Radiol, St Petersburg, Russia

[27] Univ Ghent, Dept Anat Histol & Embryol, B-9000 Ghent, Belgium

[28] Ist Super Sanita, Comparat Toxicol & Ecotoxicol Lab, I-00161 Rome, Italy

来源：

ENVIRONMENTAL AND MOLECULAR MUTAGENESIS | 2001年 / 37卷 / 01期

关键词：

cytokinesis-block micronucleus assay; peripheral blood lymphocytes; pooled re-analysis; biomarkers; DNA damage;

D O I：

暂无

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Micronucleus (MN) expression in peripheral blood lympbocytes is well established as a standard method for monitoring chromosome damage in human populations. The First results of an analysis of pooled data from laboratories using the cytokinesis-block micronucleus (CBMN) assay and participating in the HUMN (HUman MicroNucleus project) international collaborative study are presented. The effects of laboratory protocol, scoring criteria, and host factors on baseline micronucleated binucleate cell (MNC) frequency are evaluated, and a reference range of "normal" values against which future studies may be compared is provided. Primary data From historical records were submitted by 25 laboratories distributed in 16 countries. This resulted in a database of nearly 7000 subjects. Potentially significant differences were present in the methods used by participating laboratories, such as in the type of culture medium, the concentration of cytochalasin-B, the percentage of fetal calf serum, and in the culture method. Differences in criteria For scoring micronuclei were also evident. The overall median MNC frequency in nonexposed (i.e., normal) subjects was 6.5 parts per thousand and the interquartile range was between 3 and 12 parts per thousand. An increase in MNC frequency with age was evident in all but two laboratories. The effect of gender, although not so evident in all databases, was also present, with females having a 19% higher lever of MNC frequency (95% confidence interval: 14-24%). Statistical analyses were performed using random-effects models For correlated data. Our best model, which included exposure to genotoxic factors, host factors, methods, and scoring criteria, explained 75% of the total variance, with the largest contribution attributable to laboratory methods. (C) 2001 Wiley-Liss, Inc.

引用

页码：31 / 45

页数：15

共 98 条

[1] Biomarker responses in human populations: towards a worldwide map [J].