Genomic characterization of Listeria monocytogenes isolates reveals that their persistence in a pig slaughterhouse is linked to the presence of benzalkonium chloride resistance genes

被引：51

作者：

Cherifi, Tamazight ^{[1
,2
,3
]}

Carrillo, Catherine ^{[4
]}

Lambert, Dominic ^{[4
]}

Miniai, Ilhem ^{[1
,2
,3
]}

Quessy, Sylvain ^{[1
,2
,3
]}

Lariviere-Gauthier, Guillaume ^{[1
,2
,3
]}

Blais, Burton ^{[4
]}

Fravalo, Philippe ^{[1
,2
,3
]}

机构：

[1] Univ Montreal, Fac Med Vet, Chaire Rech Salubrite Viandes, St Hyacinthe, PQ, Canada

[2] Univ Montreal, Fac Med Vet, Ctr Rech Malad Infect Porcine & Avicole, St Hyacinthe, PQ, Canada

[3] Univ Montreal, Fac Med Vet, Grp Rech & Denseignement Salubrite Aliments, St Hyacinthe, PQ, Canada

[4] Canadian Food Inspect Agcy, Food Microbiol Res Team, Ottawa, ON, Canada

来源：

BMC MICROBIOLOGY | 2018年 / 18卷

基金：

加拿大自然科学与工程研究理事会;

关键词：

bcrABC cassette; Benzalkonium chloride resistance genes; Listeria monocytogenes; Pig slaughterhouse; Whole genome sequences; QUATERNARY AMMONIUM COMPOUND; BIOFILM FORMATION; PROCESSING PLANTS; STRAINS; TOLERANCE; DIVERSITY; PLASMID; GROWTH; BCRABC; PHAST;

D O I：

10.1186/s12866-018-1363-9

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

BackgroundThe aim of this study was to characterize the genomes of 30Listeria monocytogenes isolates collected at a pig slaughterhouse to determine the molecular basis for their persistence.ResultsComparison of the 30L. monocytogenes genomes showed that successive isolates (i.e., persistent types) recovered from thew sampling site could be linked on the basis of single nucleotide variants confined to prophage regions. In addition, our study revealed the presence among these strains of the bcrABC cassette which is known to produce efflux pump-mediated benzalkonium chloride resistance, and which may account for the persistence of these isolates in the slaughterhouse environment. The presence of the bcrABC cassette was confirmed by WGS and PCR and the resistance phenotype was determined by measuring minimum inhibitory concentrations. Furthermore, the BC-resistant strains were found to produce lower amounts of biofilm in the presence of sublethal concentrations of BC.ConclusionsHigh resolution SNP-based typing and determination of the bcrABC cassette may provide a means of distinguishing between resident and sporadic L. monocytogenes isolates, and this in turn will support better management of this pathogen in the food industry.

引用

页数：13

共 55 条

[1] Occurrence of and a possible mechanism for resistance to a quaternary ammonium compound in Listeria monocytogenes [J].