An Electrochemical Immunoassay for HER2 Detection

被引：78

作者：

Al-Khafaji, Q. A. M. ^{[1
,2
,3
]}

Harris, M. ^{[1
,4
]}

Tombelli, S. ^{[1
]}

Laschi, S. ^{[1
]}

Turner, A. P. F. ^{[4
,5
]}

Mascini, M. ^{[1
]}

Marrazza, G. ^{[1
]}

机构：

[1] Univ Florence, Dipartimento Chim Ugo Schiff, I-50121 Florence, Italy

[2] Al Nahrain Med Coll, Baghdad, Iraq

[3] Kamal Al Samaraei Hosp IVF & Infertil, Baghdad, Iraq

[4] Cranfield Univ, Cranfield MK43 0AL, Beds, England

[5] IFM Linkoping Univ, Biosensors & Bioelect Ctr, Linkoping, Sweden

来源：

ELECTROANALYSIS | 2012年 / 24卷 / 04期

关键词：

HER2; Cancer marker; Magnetic beads; Immunoassay; BREAST-CANCER PATIENTS; EXTRACELLULAR DOMAIN; TUMOR-MARKERS; SERUM; PROTEIN; ASSAY; AMPLIFICATION; IMMUNOSENSOR; C-ERBB-2; SURVIVAL;

D O I：

10.1002/elan.201100501

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this paper, a simple and sensitive approach for human epidermal growth factor receptor 2 (HER2) detection is presented, using antibody-functionalised magnetic beads coupled to screen-printed cells. The immunoassay is based on a sandwich format in which a primary monoclonal antibody anti-HER2 is coupled to protein A modified magnetic beads. The modified beads are then used to capture the protein from the sample solution and a sandwich assay is performed by adding a secondary monoclonal antibody anti-HER2 labelled with biotin. The enzyme alkaline phosphatase (AP) conjugated with streptavidin and its substrate (1-naphthyl-phosphate) are then used for the electrochemical detection by differential pulse voltammetry (DPV). The experimental conditions for the immunoassay were optimised. The performance of the assay in terms of sensitivity, reproducibility and selectivity has been studied in buffer and serum samples from hospital patients.

引用

页码：735 / 742

页数：8

共 21 条

[1] Comparison of different commercial kits for HER2 testing in breast cancer: looking for the accurate cutoff for amplification [J].