Establishment of a short-term storage method via encapsulation of protocorm-like bodies in Phalaenopsis bellina (Rchb.f.) Christenson

被引：2

作者：

Khoddamzadeh, A. A. ^{[1
]}

Sinniah, U. R. ^{[1
]}

Kadir, M. A. ^{[2
]}

Kadzimin, S. I. ^{[1
]}

Mahmood, M. ^{[3
]}

Subramaniam, S. ^{[4
]}

机构：

[1] Univ Putra Malaysia, Fac Agr, Dept Crop Sci, Serdang 43400, Malaysia

[2] Univ Putra Malaysia, Fac Agr, Dept Agrotechnol, Serdang 43400, Malaysia

[3] Univ Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Biochem, Serdang 43400, Malaysia

[4] Univ Sains Malaysia, Sch Biol Sci, Georgetown 11800, Penang, Malaysia

来源：

SEED SCIENCE AND TECHNOLOGY | 2011年 / 39卷 / 03期

关键词：

PROPAGATION;

D O I：

10.15258/sst.2011.39.3.19

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Protocorm-like bodies (PLBs) of phalaenopsis bellina (Rchb.f.) Christenson initiated from leaf explains, followed by proliferation on modified semi-solid, half-strength. MS media, were used in this study. PLBs measuring 3 - 4 mm in diameter were selected for encapsulation with different concentrations of sodium alginate (3, 4 and 5%) and calcium chloride (25, 50, 75 and 100 mM), with non-encapsulated PLBs as the control. The highest survival after 2 weeks incubation at 5 degrees C was 70% from the PLBs encapsulated with 4% sodium alginate plus 75 mM calcium chloride. Storage at 5, 15 and 25 degrees C for up to 60 days, led to a decline in the viability of PLBs with a greater decline when stored at higher temperature. The highest PLB fresh weight of 0.36 g also occurred after storage at 5 degrees C and the lowest fresh weight (0.19 g) occurred after 60 d at 25 degrees C when viability was 0%.

引用

页码：697 / 702

页数：6

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