Human De-etiolated-1 regulates c-Jun by assembling a CUL4A ubiquitin ligase

被引：309

作者：

Wertz, IE

O'Rourke, KM

Zhang, ZM

Dornan, D

Arnott, D

Deshaies, RJ

Dixit, VM ^{[1
]}

机构：

[1] Genentech Inc, Dept Mol Oncol, San Francisco, CA 94080 USA

[2] Genentech Inc, Dept Bioinformat, San Francisco, CA 94080 USA

[3] Genentech Inc, Dept Prot Chem, San Francisco, CA 94080 USA

[4] Univ Calif Davis, Sch Med, Dept Biol Chem, Davis, CA 95616 USA

[5] CALTECH, Howard Hughes Med Inst, Pasadena, CA 91125 USA

[6] CALTECH, Div Biol, Pasadena, CA 91125 USA

来源：

SCIENCE | 2004年 / 303卷 / 5662期

关键词：

D O I：

10.1126/science.1093549

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Arabidopsis thaliana De-etiolated-1 (AtDET1) is a highly conserved protein, with orthologs in vertebrate and invertebrate organisms. AtDET1 negatively regulates photomorphogenesis, but its biochemical mechanism and function in other species are unknown. We report that human DET1 (hDET1) promotes ubiquitination and degradation of the proto-oncogenic transcription factor c-Jun by assembling a multisubunit ubiquitin ligase containing DNA Damage Binding Protein-1 (DDB1), cullin 4A (CUL4A), Regulator of Cullins-1 (ROC1), and constitutively photomorphogenic-1. Ablation of any subunit by RNA interference stabilized c-Jun and increased c-Jun-activated transcription. These findings characterize a c-Jun ubiquitin ligase and define a specific function for hDET1 in mammalian cells.

引用

页码：1371 / 1374

页数：4

共 25 条

[1] Characterization of human constitutive photomorphogenesis protein 1, a RING finger ubiquitin ligase that interacts with Jun transcription factors and modulates their transcriptional activity [J].