Adaptive Optics Confocal Fluorescence Microscopy with Direct Wavefront Sensing for Brain Tissue Imaging

被引：0

作者：

Tao, Xiaodong ^{[1
]}

Fernandez, Bautista ^{[1
]}

Chen, Diana C. ^{[2
]}

Azucena, Oscar ^{[1
]}

Fu, Min ^{[3
]}

Zuo, Yi ^{[3
]}

Kubby, Joel ^{[1
]}

机构：

[1] Univ Calif Santa Cruz, Jack Baskin Sch Engn, 1156 High St, Santa Cruz, CA 95064 USA

[2] Lawrence Livermore Natl Lab, Livermore, CA 94550 USA

[3] Univ Calif Santa Cruz, Mol Cell & Dev Biol, Santa Cruz, CA 95064 USA

来源：

MEMS ADAPTIVE OPTICS V | 2011年 / 7931卷

基金：

美国国家科学基金会;

关键词：

Adaptive Optics; confocal microscopy; wavefront sensor; wavefront correction; deep tissue imaging; DYNAMICS;

D O I：

10.1117/12.876524

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Recently, there has been a growing interest in deep tissue imaging for the study of neurons. Unfortunately, because of the inhomogeneous refractive index of the tissue, the aberrations degrade the resolution and brightness of the final image. In this paper, we describe an adaptive optics confocal fluorescence microscope (AOCFM) which can correct aberrations based on direct wavefront measurements using a point source reference beacon and a Shack-Hartmann Wavefront Sensor (SHWS). Mouse brain tissues with different thicknesses are tested. After correction, both the signal intensity and contrast of the image are improved.

引用

页数：8

共 18 条

[1] Smart microscope: an adaptive optics learning system for aberration correction in multiphoton confocal microscopy [J].

Albert, O ;

Sherman, L ;

Mourou, G ;

Norris, TB ;

Vdovin, G .

OPTICS LETTERS, 2000, 25 (01) :52-54

[2] Wavefront aberration measurements and corrections through thick tissue using fluorescent microsphere reference beacons [J].

Azucena, Oscar ;

Crest, Justin ;

Cao, Jian ;

Sullivan, William ;

Kner, Peter ;

Gavel, Donald ;

Dillon, Daren ;

Olivier, Scot ;

Kubby, Joel .

OPTICS EXPRESS, 2010, 18 (16) :17521-17532

[3] The spatial variation of the refractive index in biological cells [J].

Beuthan, J ;

Minet, O ;

Helfmann, J ;

Herrig, M ;

Muller, G .

PHYSICS IN MEDICINE AND BIOLOGY, 1996, 41 (03) :369-382

[4] Adaptive optics in microscopy [J].

Booth, Martin J. .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES, 2007, 365 (1861) :2829-2843

[5]

Chiu CS, 2002, J NEUROSCI, V22, P10251

[6] Image-based adaptive optics for two-photon microscopy [J].

Debarre, Delphine ;

Botcherby, Edward J. ;

Watanabe, Tomoko ;

Srinivas, Shankar ;

Booth, Martin J. ;

Wilson, Tony .

OPTICS LETTERS, 2009, 34 (16) :2495-2497

[7] Long-term dendritic spine stability in the adult cortex [J].

Grutzendler, J ;

Kasthuri, N ;

Gan, WB .

NATURE, 2002, 420 (6917) :812-816

[8] Tuning FlaSh: Redesign of the dynamics, voltage range, and color of the genetically encoded optical sensor of membrane potential [J].

Guerrero, G ;

Siegel, MS ;

Roska, B ;

Loots, E ;

Isacoff, EY .

BIOPHYSICAL JOURNAL, 2002, 83 (06) :3607-3618

[9]

Ji N, 2009, NAT METHODS, V6, P179, DOI [10.1038/nmeth0209-179, 10.1038/nmeth.1411]

[10] Practical implementation of adaptive optics in multiphoton microscopy [J].

Marsh, PN ;

Burns, D ;

Girkin, JM .

OPTICS EXPRESS, 2003, 11 (10) :1123-1130

← 1 2 →