High Cytotoxicity and Apoptotic Effects of Natural Bioactive Benzofuran Derivative on the MCF-7 Breast Cancer Cell Line

被引：22

作者：

Soleimani, Afsane ^{[1
]}

Asadi, Jahanbakhsh ^{[1
]}

Rostami-Charati, Faramarz ^{[2
]}

Gharaei, Roghaye ^{[1
]}

机构：

[1] Golestan Univ Med Sci, Fac Med, Disorder Metab Res Ctr, Dept Biochem & Biophys, Golestan, Iran

[2] Gonbad Kavous Univ, Dept Chem, Fac Sci, Gonbad, Iran

来源：

COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING | 2015年 / 18卷 / 05期

关键词：

Apoptosis; benzofuran derivative; Cytotoxicity; MCF-7 breast cancer cell line; petasites hybridus; ANTIMICROBIAL ACTIVITIES; PETASITES-HYBRIDUS; DNA; ANTICANCER; ANTITUMOR; MECHANISM; TARGET; P21;

D O I：

10.2174/1386207318666150430114815

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This study was focused on evaluation of the cytotoxicity and apoptotic affects of benzofuran derivative on MCF-7 breast cancer cell line. This effective compound was isolated from the root of Petasites hybridus plant. For experiments, the MCF-7 cells were treated with several concentrations (0-500 mu M) of 1-(6-hydroxy-2-isopropenyl-1-benzofuran-5-yl)-1-ethanone 1 at different times. In this study, test of neutral red was also employed for cytotoxicity assay and quantity of P53, P21. Bax genes expression was analyzed using Real-Time PCR and ELISA techniques. Results show that compound 1 has cytotoxicity and apoptotic effects on Human breast cancer (Michigan Cancer Foundation-7) MCF-7 cells.

引用

页码：505 / 513

页数：9

共 20 条

[11] Cytostatic mechanism and antitumor potential of novel 1H-cyclopenta[b]benzofuran lignans isolated from Aglaia elliptica [J].