High Cytotoxicity and Apoptotic Effects of Natural Bioactive Benzofuran Derivative on the MCF-7 Breast Cancer Cell Line

被引：22
作者：
Soleimani, Afsane [1 ]
Asadi, Jahanbakhsh [1 ]
Rostami-Charati, Faramarz [2 ]
Gharaei, Roghaye [1 ]
机构：
[1] Golestan Univ Med Sci, Fac Med, Disorder Metab Res Ctr, Dept Biochem & Biophys, Golestan, Iran
[2] Gonbad Kavous Univ, Dept Chem, Fac Sci, Gonbad, Iran
来源：
COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING | 2015年 / 18卷 / 05期
关键词：
Apoptosis; benzofuran derivative; Cytotoxicity; MCF-7 breast cancer cell line; petasites hybridus; ANTIMICROBIAL ACTIVITIES; PETASITES-HYBRIDUS; DNA; ANTICANCER; ANTITUMOR; MECHANISM; TARGET; P21;
D O I：
10.2174/1386207318666150430114815
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
This study was focused on evaluation of the cytotoxicity and apoptotic affects of benzofuran derivative on MCF-7 breast cancer cell line. This effective compound was isolated from the root of Petasites hybridus plant. For experiments, the MCF-7 cells were treated with several concentrations (0-500 mu M) of 1-(6-hydroxy-2-isopropenyl-1-benzofuran-5-yl)-1-ethanone 1 at different times. In this study, test of neutral red was also employed for cytotoxicity assay and quantity of P53, P21. Bax genes expression was analyzed using Real-Time PCR and ELISA techniques. Results show that compound 1 has cytotoxicity and apoptotic effects on Human breast cancer (Michigan Cancer Foundation-7) MCF-7 cells.
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收藏
页码：505 / 513
页数：9
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