Amperometric detection of DNA hybridization using a multi-point, addressable electrochemical device

被引：16

作者：

Zhu, Xi ^{[2
,3
]}

Ino, Kosuke ^{[3
]}

Lin, Zhenyu ^{[2
]}

Shiku, Hitoshi ^{[3
]}

Chen, Guonan ^{[2
]}

Matsue, Tomokazu ^{[1
,3
]}

机构：

[1] Tohoku Univ, Adv Inst Mat Res, Aoba Ku, Sendai, Miyagi 9808579, Japan

[2] Fuzhou Univ, Dept Chem, Fujian Prov Key Lab Anal & Detect Food Safety, Key Lab Anal & Detect Food Safety,Minist Educ, Fuzhou 350002, Fujian, Peoples R China

[3] Tohoku Univ, Grad Sch Environm Studies, Aoba Ku, Sendai, Miyagi 9808579, Japan

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2011年 / 160卷 / 01期

关键词：

DNA detection; Addressable; Electrochemical detection; Multipoint detection; GENE-FUNCTION ANALYSIS; HIGH-DENSITY; ARRAY; MICROARRAYS; MANIPULATION;

D O I：

10.1016/j.snb.2011.09.004

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel device was designed for the multipoint addressable detection of DNA hybridization. Row and column electrodes array were orthogonally arranged, and the microwells were assembled on the crossing points of the row/column electrodes to form a 4 x 4 microwell array. Amperometric signals at the individual microwells could be detected separately on the basis of redox cycling of localized electroactive species occurring between the electrodes. Immobilization and hybridization of DNA could block the redox cycling of Fe(CN)(6)(4-)/Fe(CN)(6)(3-) at the designated microwells, resulting in the reduction of current response. This device had been used to detect DNA hybridization with excellent sensitivity (0.03 mu M) and selectivity. The device can be applied to comprehensive and high-throughput detection and imaging of biochemical species. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.

引用

页码：923 / 928

页数：6

共 32 条

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