In situ detection of rhodococci associated with activated sludge foams

被引：10

作者：

Davenport, RJ

Elliott, JN

Curtis, TP ^{[1
]}

Upton, J

机构：

[1] Univ Newcastle Upon Tyne, Dept Civil Engn, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England

[2] Nottingham Trent Univ, Dept Life Sci, Nottingham NG11 8NS, England

[3] Severn Trent Water Ltd, Proc Dev, Finham Sewage Treatment Works, Coventry CV3 6PR, W Midlands, England

来源：

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY | 1998年 / 74卷 / 1-3期

基金：

英国工程与自然科学研究理事会;

关键词：

activated sludge; foaming; fluorescent in situ hybridisation; rhodococci; mycolata;

D O I：

10.1023/A:1001795609982

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Genus-specific 16S rRNA targeted oligonucleotide probes, Rco1 and Rco2, were designed and used to detect rhodococci in activated sludge foam samples by confocal laser scanning microscopy. Pure cultures were used to find the optimal hybridisation conditions which were determined by comparing the mean fluorescent intensities of target and non-target cells from images captured using a confocal laser scanning microscope (CLSM). The combination of fluorescent in situ hybridisation with rRNA-targeted oligonucleotide probes and confocal laser scanning microscopy provides an effective way of detecting rhodococci in environmental samples.

引用

页码：41 / 48

页数：8

共 27 条

[1] FLUORESCENT-OLIGONUCLEOTIDE PROBING OF WHOLE CELLS FOR DETERMINATIVE, PHYLOGENETIC, AND ENVIRONMENTAL-STUDIES IN MICROBIOLOGY [J].