Schistosome Soluble Egg Antigen Decreases Mycobacterium tuberculosis-Specific CD4+ T-Cell Effector Function With Concomitant Arrest of Macrophage Phago-Lysosome Maturation

被引:20
作者
DiNardo, Andrew R. [1 ,2 ,4 ]
Mace, Emily M. [3 ,5 ,6 ]
Lesteberg, Kelsey [3 ,5 ,6 ]
Cirillo, Jeffrey D. [8 ]
Mandalakas, Anna M. [2 ,4 ]
Graviss, Edward A. [7 ]
Orange, Jordan S. [3 ,5 ,6 ]
Makedonas, George [3 ,5 ,6 ]
机构
[1] Texas Childrens Hosp, Dept Internal Med, Sect Infect Dis, College Stn, TX USA
[2] Texas Childrens Hosp, Dept Pediat, Immigrant & Global Hlth Program, College Stn, TX USA
[3] Texas Childrens Hosp, Baylor Coll Med, Dept Pathol & Immunol, College Stn, TX USA
[4] Texas Childrens Hosp, Global TB Program, College Stn, TX USA
[5] Texas Childrens Hosp, Dept Pediat, College Stn, TX USA
[6] Texas Childrens Hosp, Ctr Immunobiol, College Stn, TX USA
[7] Houston Methodist Hosp, Res Inst, Dept Pathol & Genom Med, College Stn, TX USA
[8] Texas A&M Hlth Sci Ctr, Ctr Airborne Pathogen Res & TB Imaging, Dept Microbial Pathogenesis & Immunol, College Stn, TX USA
基金
美国国家卫生研究院;
关键词
tuberculosis; schistosomiasis; helminth; CD4(+) T cells; T-cell function; multiparametric flow cytometry; phago-lysosome maturation; high-resolution confocal microscopy; imaging flow cytometry; ASCARIS-LUMBRICOIDES; LATENT TUBERCULOSIS; DENDRITIC CELLS; IN-VITRO; INFECTION; RESPONSES; HELMINTH; TH1; POLARIZATION; MECHANISMS;
D O I
10.1093/infdis/jiw156
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Helminth-infected individuals possess a higher risk of developing tuberculosis, but the precise immunologic mechanism of Mycobacterium tuberculosis control remains unclear. We hypothesized that a perturbation of the M. tuberculosis-specific CD4(+) T-cell response weakens the ability of macrophages to contain M. tuberculosis. We exposed peripheral blood mononuclear cells from M. tuberculosis-infected humans to schistosome soluble egg antigen (SEA) and then profiled M. tuberculosis-specific CD4(+) T cells via multiparametric flow cytometry. SEA decreased the frequency of cells producing interferon gamma (6.79% vs 3.20%; P = .017) and tumor necrosis factor a (6.98% vs 2.96%; P = .012), with a concomitant increase in the median fluorescence intensity of interleukin 4 (IL-4; P < .05) and interleukin 10 (IL-10; 1440 vs 1273; P < .05). Macrophages polarized with SEA-exposed, autologous CD4(+) T-cell supernatant had a 2.19-fold decreased colocalization of lysosomes and M. tuberculosis (P < .05). When polarized with IL-4 or IL-10, macrophages had increased expression of CD206 (P < .0001), 1.5-fold and 1.9 fold increased intracellular numbers of M. tuberculosis per macrophage (P < .0005), and 1.4-fold and 1.7-fold decreased colocalization between M. tuberculosis and lysosomes (P < .001). This clarifies a relationship in which helminth-induced CD4(+) T cells disrupt M. tuberculosis control by macrophages, thereby providing a mechanism for the observation that helminth infection advances the progression of tuberculosis among patients with M. tuberculosis infection.
引用
收藏
页码:479 / 488
页数:10
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