SugE, a New Member of the SMR Family of Transporters, Contributes to Antimicrobial Resistance in Enterobacter cloacae

被引：44

作者：

He, Gui-Xin ^{[1
]}

Zhang, Chu ^{[1
]}

Crow, Robert R. ^{[2
]}

Thorpe, Conner ^{[1
]}

Chen, Huizhong ^{[3
]}

Kumar, Sanath ^{[2
]}

Tsuchiya, Tomofusa ^{[4
]}

Varela, Manuel F. ^{[2
]}

机构：

[1] Univ Massachusetts Lowell, Dept Clin Lab & Nutr Sci, Lowell, MA 01854 USA

[2] Eastern New Mexico Univ, Dept Biol, Portales, NM 88130 USA

[3] US FDA, Div Microbiol, Natl Ctr Toxicol Res, Jefferson, AR 72079 USA

[4] Okayma Univ, Dept Mol Microbiol, Okayama 7008530, Japan

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2011年 / 55卷 / 08期

关键词：

MULTIDRUG EFFLUX PUMP; PSEUDOMONAS-AERUGINOSA; SUSCEPTIBILITY; CLONING; SYSTEM; STRAIN; ACRAB;

D O I：

10.1128/AAC.00094-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We cloned a gene, sugE, from the chromosome of Enterobacter cloacae ATCC 13047. Analysis of the susceptibilities of the sugE-containing strain (Escherichia coli KAM32/pSUGE28) and sugE-deficient E. cloacae (Ec Delta sugE) showed that SugE confers resistance to cetyltrimethylammonium bromide, cetylpyridinium chloride, tetraphenylphosphonium, benzalkonium chloride, ethidium bromide, and sodium dodecyl sulfate. We also investigated expression of sugE. We confirm here that SugE from E. cloacae is an SMR family transporter as determined by observing its energy-dependent drug efflux activity.

引用

页码：3954 / 3957

页数：4

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