HPMC-PVA Film Immobilized Rhizopus oryzae Lipase as a Biocatalyst for Transesterification Reaction

被引：55

作者：

Dhake, Kishor P. ^{[1
]}

Tambade, Pawan J. ^{[1
]}

Qureshi, Ziyauddin S. ^{[1
]}

Singhal, Rekha S. ^{[2
]}

Bhanage, Bhalchandra M. ^{[1
]}

机构：

[1] Inst Chem Technol Autonomous, Dept Chem, Bombay 400019, Maharashtra, India

[2] Inst Chem Technol Autonomous, Dept Food Engn & Technol, Bombay 400019, Maharashtra, India

来源：

ACS CATALYSIS | 2011年 / 1卷 / 04期

关键词：

Rhizopus oryzae lipase; immobilization; acetate synthesis; transesterification; polymers; biocatalysis; CANDIDA-RUGOSA LIPASE; CATALYZED SYNTHESIS; VINYL-ACETATE; SUPPORT; STABILITY; ALCOHOLS; ENZYMES;

D O I：

10.1021/cs100162t

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The film prepared using a blend of hydroxypropyl methyl cellulose (HPMC) and polyvinyl alcohol (PVA) was investigated for immobilization of Rhizopus oryzae lipase. These immobilized lipase films were characterized using a combination of techniques like SEM, TGA, FT-IR, and Karl Fischer titration analysis. The biocatalyst was subjected to transesterification reaction of benzyl alcohol with vinyl acetate and was optimized for various reaction parameters such as effect of support, molar ratio, solvent, concentration of biocatalyst, time and temperature. Furthermore, the developed methodology was then effectively applied to various alcohols for synthesis of industrially important acetates providing good to excellent yields of desired products. Interestingly, the experimental results demonstrated catalytic activity of immobilized lipase to be 4-fold greater than that of free lipase for transesterification reaction. The immobilized biocatalyst was effectively recycled for four consecutive cycles and exhibited remarkable stability for a period 90 days.

引用

页码：316 / 322

页数：7

共 34 条

[1] Carboxymethylcellulose and poly(vinyl alcohol) used as a film support for lipases immobilization [J].

Dalla-Vecchia, R ;

Sebrao, D ;

Nascimento, MD ;

Soldi, V .

PROCESS BIOCHEMISTRY, 2005, 40 (08) :2677-2682

[2] Biocatalysis and enzymes in organic synthesis [J].

Davis, BG ;

Borer, V .

NATURAL PRODUCT REPORTS, 2001, 18 (06) :618-640

[3] Promiscuous Candida antarctica lipase B-catalyzed synthesis of β-amino esters via aza-Michael addition of amines to acrylates [J].

Dhake, Kishor P. ;

Tambade, Pawan J. ;

Singhal, Rekha S. ;

Bhanage, Bhalchandra M. .

TETRAHEDRON LETTERS, 2010, 51 (33) :4455-4458

[4] Candida antarctica lipase B-catalyzed synthesis of acetamides using [BMIm(PF6)] as a reaction medium [J].

Dhake, Kishor P. ;

Qureshi, Ziyauddin S. ;

Singhal, Rekha S. ;

Bhanage, Bhalchandra M. .

TETRAHEDRON LETTERS, 2009, 50 (23) :2811-2814

[5] Solid trichlorotitanium(IV) trifluoromethanesulfonate TiCl3(OTf) catalyzed efficient acylation of -OH and -SH:: Direct esterification of alcohols with carboxylic acids and transesterification of alcohols with esters under neat conditions [J].

Firouzabadi, Habib ;

Iranpoor, Nasser ;

Farahi, Soghra .

JOURNAL OF MOLECULAR CATALYSIS A-CHEMICAL, 2008, 289 (1-2) :61-68

[6] Effect of pore diameter and cross-linking method on the immobilization efficiency of Candida rugosa lipase in SBA-15 [J].

Gao, Siliang ;

Wang, Yujun ;

Diao, Xiang ;

Luo, Guangsheng ;

Dai, Youyuan .

BIORESOURCE TECHNOLOGY, 2010, 101 (11) :3830-3837

[7] Immobilization of lipase on methyl-modified silica aerogels by physical adsorption [J].

Gao, Siliang ;

Wang, Yujun ;

Wang, Tao ;

Luo, Guangsheng ;

Dai, Youyuan .

BIORESOURCE TECHNOLOGY, 2009, 100 (02) :996-999

[8]

GOODSHIP V, 2009, POLYVINYL ALCOHOL MA, V16, P1

[9] Understanding enzyme immobilisation [J].

Hanefeld, Ulf ;

Gardossi, Lucia ;

Magner, Edmond .

CHEMICAL SOCIETY REVIEWS, 2009, 38 (02) :453-468

[10] Immobilised Burkholderia cepacia lipase in dry organic solvents and ionic liquids: A comparison [J].

Hara, Piia ;

Hanefeld, Ulf ;

Kanerv, Liisa T. .

GREEN CHEMISTRY, 2009, 11 (02) :250-256

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