Production and characterization of virus-like particles and the P domain protein of GII.4 norovirus

被引：36

作者：

Koho, Tiia ^{[1
,2
]}

Huhti, Leena ^{[3
]}

Blazevic, Vesna ^{[3
]}

Nurminen, Kirsi ^{[3
]}

Butcher, Sarah J. ^{[4
]}

Laurinmaki, Pasi ^{[4
]}

Kalkkinen, Nisse ^{[4
]}

Ronnholm, Gunilla ^{[4
]}

Vesikari, Timo ^{[3
]}

Hytonen, Vesa P. ^{[1
,2
]}

Kulomaa, Markku S. ^{[1
,2
]}

机构：

[1] Univ Tampere, Inst Biomed Technol & BioMediTech, FI-33014 Tampere, Finland

[2] Univ Tampere, Tampere Univ Hosp, FI-33014 Tampere, Finland

[3] Univ Tampere, Vaccine Res Ctr, FI-33014 Tampere, Finland

[4] Univ Helsinki, Inst Biotechnol, FI-00014 Helsinki, Finland

来源：

JOURNAL OF VIROLOGICAL METHODS | 2012年 / 179卷 / 01期

基金：

芬兰科学院;

关键词：

Virus-like particle; VLP; Norovirus; P domain protein; Diagnostics; GROUP ANTIGEN RECEPTORS; BLOOD GROUP ANTIGENS; NORWALK VIRUS; CAPSID PROTEIN; GASTROENTERITIS; FORMS; CLASSIFICATION; RECOGNITION; EXPRESSION; STABILITY;

D O I：

10.1016/j.jviromet.2011.05.009

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Noroviruses are an important cause of epidemic acute gastroenteritis in humans. In this study the production and characterization of GII.4 norovirus virus-like particles (VLPs) in insect cells is reported. Furthermore, the expression of corresponding norovirus polyhistidine-tagged P domain protein in Escherichia coli is described. The protruding P domain of the norovirus capsid is known to contain determinants for antibody and receptor binding. Therefore, P domain proteins were studied as an alternative diagnostic tool for evaluating norovirus infection. Analyses by dynamic light scattering and cryo-electron microscopy revealed the presence of intact VLPs with an average diameter of about 40 nm. Immunostaining and ELISA assays using norovirus-specific human sera revealed that VLPs and the P domain are recognized by norovirus-specific antibodies and by their putative receptor. The VLPs and P domain protein are potentially useful in the development of diagnostic and vaccination tools for noroviruses. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：1 / 7

页数：7

共 35 条

[1] WINTER VOMITING DISEASE [J].

ADLER, JL ;

ZICKL, R .

JOURNAL OF INFECTIOUS DISEASES, 1969, 119 (06) :668-+

[2] Conformational stability and disassembly of Norwalk virus-like particles - Effect of pH and temperature [J].

Ausar, Salvador F. ;

Foubert, Thomas R. ;

Hudson, Mary H. ;

Vedvick, Thomas S. ;

Middaugh, C. Russell .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (28) :19478-19488

[3] Adding the third dimension to virus life cycles: Three-dimensional reconstruction of icosahedral viruses from cryo-electron micrographs [J].

Baker, TS ;

Olson, NH ;

Fuller, SD .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1999, 63 (04) :862-+

[4] The 3′ end of Norwalk virus mRNA contains determinants that regulate the expression and stability of the viral capsid protein VP1:: a novel function for the VP2 protein [J].

Bertolotti-Ciarlet, A ;

Crawford, SE ;

Hutson, AM ;

Estes, MK .

JOURNAL OF VIROLOGY, 2003, 77 (21) :11603-11615

[5] Structural requirements for the assembly of Norwalk virus-like particles [J].

Bertolotti-Ciarlet, A ;

White, LJ ;

Chen, R ;

Prasad, BVV ;

Estes, MK .

JOURNAL OF VIROLOGY, 2002, 76 (08) :4044-4055

[6] PHOTON CORRELATION STUDY OF POLYDISPERSE SAMPLES OF POLYSTYRENE IN CYCLOHEXANE [J].

BROWN, JC ;

PUSEY, PN ;

DIETZ, R .

JOURNAL OF CHEMICAL PHYSICS, 1975, 62 (03) :1136-1144

[7] Molecular epidemiology of caliciviruses causing outbreaks and sporadic cases of acute gastroenteritis in Spain [J].

Buesa, J ;

Collado, B ;

López-Andújar, P ;

Abu-Mallouh, R ;

Díaz, JR ;

Díaz, AG ;

Prat, J ;

Guix, S ;

Llovet, T ;

Prats, G ;

Bosch, A .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (08) :2854-2859

[8] Structural basis for the recognition of blood group trisaccharides by norovirus [J].

Cao, Sheng ;

Lou, Zhiyong ;

Tan, Ming ;

Chen, Yutao ;

Liu, Yijin ;

Zhang, Zhushan ;

Zhang, Xuejun C. ;

Jiang, Xi ;

Li, Xuemei ;

Rao, Zihe .

JOURNAL OF VIROLOGY, 2007, 81 (11) :5949-5957

[9] Atomic resolution structural characterization of recognition of histo-blood group antigens by Norwalk virus [J].

Choi, Jae-Mun ;

Hutson, Anne M. ;

Estes, Mary K. ;

Prasad, B. V. Venkataram .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (27) :9175-9180

[10] Epidemiologic and molecular trends of "Norwalk-like viruses" associated with outbreaks of gastroenteritis in the United States [J].

Fankhauser, RL ;

Monroe, SS ;

Noel, JS ;

Humphrey, CD ;

Bresee, JS ;

Parashar, UD ;

Ando, T ;

Glass, RI .

JOURNAL OF INFECTIOUS DISEASES, 2002, 186 (01) :1-7

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