Effect of Cu-nanoparticles versus one Cu-salt: Analysis of stress biomarkers response in Enchytraeus albidus (Oligochaeta)

被引:56
作者
Gomes, Susana I. L. [1 ,2 ]
Novais, Sara C. [1 ,2 ]
Gravato, Carlos [3 ,4 ,5 ]
Guilhermino, Lucia [3 ,4 ,5 ]
Scott-Fordsmand, Janeck J. [6 ]
Soares, Amadeu M. V. M. [1 ,2 ]
Amorim, Monica J. B. [1 ,2 ]
机构
[1] Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal
[2] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal
[3] Univ Porto, CIMAR LA, CIIMAR, P-4100 Oporto, Portugal
[4] Univ Porto, ICBAS Ctr Interdisciplinar Invest Marinha & Ambie, Lab Ecotoxicol, P-4100 Oporto, Portugal
[5] Univ Porto, ICBAS, Lab Ecotoxicol, P-4100 Oporto, Portugal
[6] Aarhus Univ, Dept Terr Ecol, Natl Environm Res Inst, Vejlsovej, Denmark
关键词
Oxidative stress; enzymatic and non-enzymatic antioxidants; copper nanoparticles; copper; Enchytraeids; COPPER NANOPARTICLES; OXIDATIVE STRESS; LIPID-PEROXIDATION; DAPHNIA-MAGNA; GLUTATHIONE-REDUCTASE; SUPEROXIDE-DISMUTASE; TITANIUM-DIOXIDE; DIGESTIVE GLAND; SOIL PROPERTIES; FULLERENE C-60;
D O I
10.3109/17435390.2011.562327
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
In the present study, the main goal was to compare the effects of ionic copper versus copper nanoparticles in Enchytraeus albidus assessing the effect at the biomarker level, testing different concentrations and exposure times. Measured parameters were lipid peroxidation (LPO), total, reduced and oxidized glutathione content (TG, GSH and GSSG), the enzymatic activity of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx), glutathione S-transferases (GSTs) and cholinesterases (ChEs). Results showed that both salt-and nano-copper caused oxidative stress and damage to E. albidus, as confirmed by LPO levels, and effects could be discriminated between the copper forms. Nevertheless and despite the visible discrimination between nano and the salt form (time and exposure dependent), there was no single or a set of biomarkers that provided the best discrimination.
引用
收藏
页码:134 / 143
页数:10
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