Induction of alternative oxidase chain under salt stress conditions

被引:18
作者
Ferreira, A. L. [1 ]
Arrabaca, J. D. [2 ]
Vaz-Pinto, V. [2 ]
Lima-Costa, M. E.
机构
[1] Univ Algarve, Fac Engn Recursos Nat, P-8005134 Gambelas, Portugal
[2] Univ Lisbon, Fac Ciencias, P-1749016 Lisbon, Portugal
关键词
cell suspension; Citrus; respiration rate;
D O I
10.1007/s10535-008-0009-4
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
This paper describes the effect of NaCl on the respiration of Citrus cell suspensions namely on the induction of the alternative oxidase. The exposure of two Citrus (cvs. Carvalhal tangor and Valencia late) cell suspensions to 200 or 400 mM NaCl lead to a reduction on cell respiration rates. Under these conditions, the respiration rate decreased less in the presence of KCN indicating a stimulation of the capacity of the alternative oxidase (AOX). In addition, immunoblots showed an increase on the amount of AOX protein. Antibodies raised against the Sauromatum guttatum enzyme recognized the reduced form of the enzyme near the 35 kDa band. The protein accumulation was correlated with the significantly higher AOX capacity observed for cv. Carvalhal tangor.
引用
收藏
页码:66 / 71
页数:6
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