A comparative evaluation of genetic material from Quercus robur L. stem tissues of different age and living states

Qualitative and quantitative evaluation of the total DNA extracts from the steams tissues of different morphological locations was performed for ecologically and economically important wood tree species pedunculate oak (Quercus robur L.). Generally, the younger plant leaves are known to have a significantly higher content of the undegraded DNA. However, sometimes it is difficult to collect them practically. It follows that qualitative and quantitative DNA isolation may require other organs and tissues of plants, such as the stems. Three types of primers, i.e., nuclear DNA degenerate ISSR-primer UBC 841; chloroplast DNA-primers complementary to the gene fragment (rbcl_a) of a large subunit of 1,5-ribulose-bisphosphate carboxylase/oxygenase (RuBisCO) and mitochondrial DNA-primers targeting the gene encoding ATPase 1 (atp 1) were used for the qualitative evaluation of total DNA extraction. The highest concentration of total DNA was found in the phloem and cambium (35.23 ng/mu l for the wood cuts from living plants and 12.62 ng/mu l for the wood cuts of dead plants) obtained from the Q. robur L. stems. Successful enzymatic amplification of the isolated DNA was achieved only for the objects from the locations related to the cambium, bast, and sapwoods tissues of stems. Generally, it has been shown that Q. robur L. stem can be used for the obtaining of an acceptable concentrations of a good quality DNA. The results of the current study are presented for the first time to make the practical recommendations for sampling the tissues from a living woody tree with the subsequent PCR analysis.