Generation of two ERF gene knockout human embryonic stem cell lines using CRISPR/Cas9 system

被引：1

作者：

Si, Lihui ^{[1
]}

Yang, Ruiqi ^{[1
]}

Liu, Jian ^{[1
]}

Dong, Yubo ^{[2
]}

Zhang, Hongtao ^{[2
]}

Xu, Xiaohong ^{[1
]}

机构：

[1] Second Hosp Jilin Univ, Dept Obstet & Gynecol, Changchun, Jilin, Peoples R China

[2] Meihekou Cent Hosp, Meihekou, Jilin, Peoples R China

来源：

STEM CELL RESEARCH | 2019年 / 41卷

关键词：

D O I：

10.1016/j.scr.2019.101644

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Human ERF gene is a transcription factor involved in development, trophoblast differentiation, apoptosis, and cancer progress. To further understand the exact roles of ERF in these processes, here we report that establishment of two ERF knockout human embryonic stem cell (hESC) lines by CRISPR/Cas9 mediated gene targeting. These cell lines exhibited classical hESC morphology and normal karyotype, and highly expressed pluripotent markers, and had differentiation potential in vitro. These cell lines provide good materials to understand the roles of ERF in development, trophoblast differentiation and craniosynostosis for further studies.

引用

页数：4

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