L-carbocisteine inhibits respiratory syncytial virus infection in human tracheal epithelial cells

被引:12
作者
Asada, Masanori [2 ]
Yoshida, Motoki [2 ]
Hatachi, Yukimasa [3 ]
Sasaki, Takahiko [4 ]
Yasuda, Hiroyasu [5 ]
Deng, Xue [5 ]
Nishimura, Hidekazu [6 ]
Kubo, Hiroshi [1 ]
Nagatomi, Ryoichi
Yamaya, Mutsuo [1 ]
机构
[1] Tohoku Univ, Grad Sch Med, Dept Adv Prevent Med Infect Dis, Aoba Ku, Sendai, Miyagi 9808575, Japan
[2] Sendai City Hosp, Dept Infect Dis, Sendai, Miyagi, Japan
[3] Kyoto Univ, Grad Sch Med, Dept Resp Med, Kyoto, Japan
[4] Tohoku Univ, Sch Med, Dept Resp Med, Sendai, Miyagi 9808575, Japan
[5] Tohoku Univ, Sch Med, New Biomed Engn Ctr, Dept Innovat, Sendai, Miyagi 9808575, Japan
[6] Sendai Natl Hosp, Div Clin Res, Virus Res Ctr, Sendai, Miyagi, Japan
关键词
Bronchial asthma; Carbocisteine; COPD; ICAM-1; Respiratory syncytial virus; OBSTRUCTIVE PULMONARY-DISEASE; LYSOPHOSPHATIDIC ACID; RHINOVIRUS INFECTION; ADHESION; BINDING; RHO; GLYCOSAMINOGLYCANS; GLYCOPROTEIN; KINASE;
D O I
10.1016/j.resp.2011.10.017
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
To examine the effects of L-carbocisteine on airway infection with respiratory syncytial (RS) virus, human tracheal epithelial cells were pretreated with L-carbocisteine and infected with RS virus. Viral titer, virus RNA, and pro-inflammatory cytokine secretion, including interleukin (IL)-1 and IL-6, increased with time after infection. L-carbocisteine reduced the viral titer in the supernatant fluids, the amount of RS virus RNA. RS virus infection susceptibility, and the concentration of pro-inflammatory cytokines induced by virus infection. L-carbocisteine reduced the expression of intercellular adhesion molecule (ICAM)-1, an RS virus receptor, on the cells. However, L-carbocisteine had no effects on the expression of heparan sulfate, a glycosaminoglycan that binds to the RS virus attachment protein, or on the amount of intracellular activated-RhoA, isoform A of the Ras-homologous family, that binds to the RS virus fusion protein. These findings suggest that L-carbocisteine may inhibit RS virus infection by reducing the expression of ICAM-1. It may also modulate airway inflammation during RS virus infection. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:112 / 118
页数:7
相关论文
共 34 条
[31]   Procaterol inhibits rhinovirus infection in primary cultures of human tracheal epithelial cells [J].
Yamaya, Mutsuo ;
Nishimura, Hidekazu ;
Hatachi, Yukimasa ;
Yoshida, Motoki ;
Fujiwara, Hidenori ;
Asada, Masanori ;
Nakayama, Katsutoshi ;
Yasuda, Hiroyasu ;
Deng, Xue ;
Sasaki, Takahiko ;
Kubo, Hiroshi ;
Nagatomi, Ryoichi .
EUROPEAN JOURNAL OF PHARMACOLOGY, 2011, 650 (01) :431-444
[32]   Carbocisteine inhibits rhinovirus infection in human tracheal epithelial cells [J].
Yasuda, H. ;
Yamaya, M. ;
Sasaki, T. ;
Inoue, D. ;
Nakayama, K. ;
Yamada, M. ;
Asada, M. ;
Yoshida, M. ;
Suzuki, T. ;
Nishimura, H. ;
Sasaki, H. .
EUROPEAN RESPIRATORY JOURNAL, 2006, 28 (01) :51-58
[33]   Carbocisteine reduces frequency of common colds and exacerbations in patients with chronic obstructive pulmonary disease [J].
Yasuda, H ;
Yamaya, M ;
Sasaki, T ;
Inoue, D ;
Nakayama, K ;
Tomita, N ;
Yoshida, M ;
Sasaki, H .
JOURNAL OF THE AMERICAN GERIATRICS SOCIETY, 2006, 54 (02) :378-380
[34]   Effect of carbocisteine on acute exacerbation of chronic obstructive pulmonary disease (PEACE Study): a randomised placebo-controlled study [J].
Zheng, Jin-Ping ;
Kang, Jian ;
Huang, Shao-Guang ;
Chen, Ping ;
Yao, Wan-Zen ;
Yang, Lan ;
Bai, Chun-Xue ;
Wang, Chang-Zheng ;
Wang, Chen ;
Chen, Bao-Yuan ;
Shi, Yi ;
Liu, Chun-Tao ;
Chen, Ping ;
Li, Qiang ;
Wang, Zhen-Shan ;
Huang, Yi-Jiang ;
Luo, Zhi-Yang ;
Chen, Fei-Peng ;
Yuan, Jian-Zhang ;
Yuan, Ben-Tong ;
Qian, Hui-Ping ;
Zhi, Rong-Chang ;
Zhong, Nan-Shan .
LANCET, 2008, 371 (9629) :2013-2018