Pathogenicity and pathogenesis of a United States porcine deltacoronavirus cell culture isolate in 5-day-old neonatal piglets

被引：155

作者：

Chen, Qi ^{[1
]}

Gauger, Phillip ^{[1
]}

Stafne, Molly ^{[1
]}

Thomas, Joseph ^{[1
]}

Arruda, Paulo ^{[1
]}

Burrough, Eric ^{[1
]}

Madson, Darin ^{[1
]}

Brodie, Joseph ^{[1
]}

Magstadt, Drew ^{[1
]}

Derscheid, Rachel ^{[1
]}

Welch, Michael ^{[1
]}

Zhang, Jianqiang ^{[1
]}

机构：

[1] Iowa State Univ, Dept Vet Diagnost & Prod Anim Med, Ames, IA 50011 USA

来源：

VIROLOGY | 2015年 / 482卷

关键词：

PDCoV; Coronavirus; Atrophic enteritis; Peptide-specific antisera; Immunohistochemistry; EPIDEMIC DIARRHEA VIRUS; EXPERIMENTAL-INFECTION; AVIAN CORONAVIRUSES; PIGS;

D O I：

10.1016/j.virol.2015.03.024

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Porcine deltacoronavirus (PDCoV) was first identified in Hong Kong in 2009-2010 and reported in United States swine for the first time in February 2014. However, diagnostic tools other than polymerase chain reaction for PDCoV detection were lacking and Koch's postulates had not been fulfilled to confirm the pathogenic potential of PDCoV. In the present study, PDCoV peptide-specific rabbit antisera were developed and used in immunofluorescence and immunohistochemistry assays to assist PDCoV diagnostics. The pathogenicity and pathogenesis of PDCoV was investigated following orogastric inoculation of 5-day-old piglets with a plaque-purified PDCoV cell culture isolate (3 x 10(4) TCID50 per pig). The PDCoV-inoculated piglets developed mild to moderate diarrhea, shed increasing amount of virus in rectal swabs from 2 to 7 days post inoculation, and developed macroscopic and microscopic lesions in small intestines with viral antigen confirmed by immunohistochemistry staining. This study experimentally confirmed PDCoV pathogenicity and characterized PDCoV pathogenesis in neonatal piglets. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：51 / 59

页数：9

共 17 条

[1] Isolation and Characterization of Porcine Epidemic Diarrhea Viruses Associated with the 2013 Disease Outbreak among Swine in the United States [J].

Chen, Qi ;

Li, Ganwu ;

Stasko, Judith ;

Thomas, Joseph T. ;

Stensland, Wendy R. ;

Pillatzki, Angela E. ;

Gauger, Phillip C. ;

Schwartz, Kent J. ;

Madson, Darin ;

Yoon, Kyoung-Jin ;

Stevenson, Gregory W. ;

Burrough, Eric R. ;

Harmon, Karen M. ;

Main, Rodger G. ;

Zhang, Jianqiang .

JOURNAL OF CLINICAL MICROBIOLOGY, 2014, 52 (01) :234-243

[2] THE PATHOGENESIS OF AN ENTERIC INFECTION IN PIGS, EXPERIMENTALLY INDUCED BY THE CORONAVIRUS-LIKE AGENT, CV-777 [J].

DEBOUCK, P ;

PENSAERT, M ;

COUSSEMENT, W .

VETERINARY MICROBIOLOGY, 1981, 6 (02) :157-165

[3] Detection of a novel and highly divergent coronavirus from Asian leopard cats and Chinese ferret badgers in southern China [J].

Dong, B. Q. ;

Liu, W. ;

Fan, X. H. ;

Vijaykrishna, D. ;

Tang, X. C. ;

Gao, F. ;

Li, L. F. ;

Li, G. J. ;

Zhang, J. X. ;

Yang, L. Q. ;

Poon, L. L. M. ;

Zhang, S. Y. ;

Peiris, J. S. M. ;

Smith, G. J. D. ;

Chen, H. ;

Guan, Y. .

JOURNAL OF VIROLOGY, 2007, 81 (13) :6920-6926

[4] Pathology of US Porcine Epidemic Diarrhea Virus Strain PC21A in Gnotobiotic Pigs [J].

Jung, Kwonil ;

Wang, Qiuhong ;

Scheuer, Kelly A. ;

Lu, Zhongyan ;

Zhang, Yan ;

Saif, Linda J. .

EMERGING INFECTIOUS DISEASES, 2014, 20 (04) :662-665

[5] Experimental infection of piglets with transmissible gastroenteritis virus: a comparison of three strains (Korean, Purdue and Miller) [J].

Kim, B ;

Chae, C .

JOURNAL OF COMPARATIVE PATHOLOGY, 2002, 126 (01) :30-37

[6] Experimental infection of piglets with a Korean strain of porcine epidemic diarrhoea virus [J].

Kim, O ;

Chae, C .

JOURNAL OF COMPARATIVE PATHOLOGY, 2003, 129 (01) :55-60

[7]

Lee S., 2014, GENOME ANNOUNC, V2, P6

[8] Full-Length Genome Sequence of Porcine Deltacoronavirus Strain USA/IA/2014/8734 [J].

Li, Ganwu ;

Chen, Qi ;

Harmon, Karen M. ;

Yoon, Kyoung-Jin ;

Schwartz, Kent J. ;

Hoogland, Marlin J. ;

Gauger, Phillip C. ;

Main, Rodger G. ;

Zhang, Jianqiang .

GENOME ANNOUNCEMENTS, 2014, 2 (02)

[9]

Madson D., 2015, VET PATHOL IN PRESS

[10] Pathogenesis of porcine epidemic diarrhea virus isolate (US/Iowa/18984/2013) in 3-week-old weaned pigs [J].

Madson, D. M. ;

Magstadt, D. R. ;

Arruda, P. H. E. ;

Hoang, H. ;

Sun, D. ;

Bower, L. P. ;

Bhandari, M. ;

Burrough, E. R. ;

Gauger, P. C. ;

Pillatzki, A. E. ;

Stevenson, G. W. ;

Wilberts, B. L. ;

Brodie, J. ;

Harmon, K. M. ;

Wang, C. ;

Main, R. G. ;

Zhang, J. ;

Yoon, K. J. .

VETERINARY MICROBIOLOGY, 2014, 174 (1-2) :60-68

← 1 2 →