Rapid multiplex polymerase chain reaction for detection of mcr-1 to mcr-5 genes

被引：54

作者：

Lescat, Mathilde ^{[1
,2
,3
,4
]}

Poirel, Laurent ^{[1
,2
,5
]}

Nordmann, Patrice ^{[1
,2
,5
,6
,7
]}

机构：

[1] Univ Fribourg, Dept Med, Med & Mol Microbiol, Emerging Antibiot Resistance Unit, Fribourg, Switzerland

[2] IAME, LEA, INSERM European Unit, Paris, France

[3] Univ Paris Nord, Sorbonne Paris Cite, Paris, France

[4] Hop Univ Paris Seine St Denis, AP HP, Bondy, France

[5] Univ Fribourg, Swiss Natl Reference Ctr Emerging Antibiot Resist, Fribourg, Switzerland

[6] Univ Lausanne, Lausanne, Switzerland

[7] Univ Hosp Ctr, Lausanne, Switzerland

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2018年 / 92卷 / 04期

基金：

瑞士国家科学基金会;

关键词：

Polymyxin resistance6; mcr genes; Multiplex PCR; REAL-TIME PCR; ESCHERICHIA-COLI; KLEBSIELLA-PNEUMONIAE; RESISTANCE; BACTERIA; PLASMID;

D O I：

10.1016/j.diagmicrobio.2018.04.010

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

A rapid (total time <2 h) and reliable multiplex polymerase chain reaction for screening of mcr-1 to mcr-5 genes conferring resistance to colistin has been developed. This technique has been tested on a collection of isolates previously identified as bearing mcr-1, mcr-2, and mcr-like genes and had a sensitivity and a specificity of 100%. Using this method, we were also able to identify a single isolate possessing both mcr-1 and mcr-5 genes. (C) 2018 Published by Elsevier Inc.

引用

页码：267 / 269

页数：3

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