Genome-wide identification and expression profiling of the YUCCA gene family in Malus domestica

被引：16

作者：

Song, Chunhui ^{[1
,2
]}

Zhang, Dong ^{[1
]}

Zheng, Liwei ^{[1
]}

Shen, Yawen ^{[1
]}

Zuo, Xiya ^{[1
]}

Mao, Jiangping ^{[1
]}

Meng, Yuan ^{[1
]}

Wu, Haiqin ^{[4
]}

Zhang, Yike ^{[5
]}

Liu, Xiaoyuan ^{[6
]}

Qian, Ming ^{[1
]}

Zhang, Jie ^{[7
]}

Li, Gaochao ^{[1
]}

Zhao, Caiping ^{[1
]}

Xing, Libo ^{[1
]}

Ma, Juanjuan ^{[1
]}

Han, Mingyu ^{[1
]}

An, Na ^{[1
,3
]}

机构：

[1] Northwest A&F Univ, Coll Hort, Yangling 712100, Shaanxi, Peoples R China

[2] Henan Agr Univ, Coll Hort, Zhengzhou 450002, Henan, Peoples R China

[3] Northwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China

[4] Gaotou Township Peoples Govt Wuji Cty, Shijiazhuang 050000, Hebei, Peoples R China

[5] Taiyuan Acad Agr Sci, Taiyuan 030000, Peoples R China

[6] Weinan Agrotech Extens Ctr, Weinan 714000, Shaanxi, Peoples R China

[7] Silkworm Tea Fruit Technol Extens Ctr Nanzheng Di, Hanzhong 723100, Shaanxi, Peoples R China

来源：

SCIENTIFIC REPORTS | 2020年 / 10卷 / 01期

基金：

中国国家自然科学基金;

关键词：

FLAVIN MONOOXYGENASE GENE; AUXIN BIOSYNTHESIS; ABSCISIC-ACID; ARABIDOPSIS; LEAF; PURIFICATION; RESISTANCE; TISSUES; ENZYME;

D O I：

10.1038/s41598-020-66483-y

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The plant hormone auxin is essential for plant growth and development. YUCCA proteins catalyse the rate-limiting step for endogenous auxin biosynthesis. In this study, we isolated 20 MdYUCCA genes from apple genome. MdYUCCA6a, MdYUCCA8a, and MdYUCCA10a were expressed in most organs and could support whole plant basal auxin synthesis. MdYUCCA4a, MdYUCCA10b, and MdYUCCA11a expression indicated roles for these genes in auxin biosynthesis in vegetative organs. MdYUCCA2b, MdYUCCA11b, and MdYUCCA11d were mainly expressed in flower organs. High temperature induced the expression of MdYUCCA4a, MdYUCCA6a, MdYUCCA8a, and MdYUCCA10a, and down-regulated the expression of MdYUCCA2b and MdYUCCA6b. Dual-luciferase assay indicated that MdPIF4 could trans-activate the MdYUCCA8a promoter. Overexpression of MdYUCCA8a increased IAA content, increased stem height, enhanced apical dominance, and led to silique malformation. These results provide a foundation for further investigation of the biological functions of apple MdYUCCAs.

引用

页数：12

共 43 条

[1]

[Anonymous], 2007, CSH PROTOC

[2] A novel thiol-reductase activity of Arabidopsis YUC6 confers drought tolerance independently of auxin biosynthesis [J].

Cha, Joon-Yung ;

Kim, Woe-Yeon ;

Kang, Sun Bin ;

Kim, Jeong Im ;

Baek, Dongwon ;

Jung, In Jung ;

Kim, Mi Ri ;

Li, Ning ;

Kim, Hyun-Jin ;

Nakajima, Masatoshi ;

Asami, Tadao ;

Sabir, Jamal S. M. ;

Park, Hyeong Cheol ;

Lee, Sang Yeol ;

Bohnert, Hans J. ;

Bressan, Ray A. ;

Pardo, Jose M. ;

Yun, Dae-Jin .

NATURE COMMUNICATIONS, 2015, 6

[3]

Chang S. J., 1993, Plant Molecular Biology Reporter, V11, P113, DOI 10.1007/BF02670468

[4] Auxin Overproduction in Shoots Cannot Rescue Auxin Deficiencies in Arabidopsis Roots [J].

Chen, Qingguo ;

Dai, Xinhua ;

De-Paoli, Henrique ;

Cheng, Youfa ;

Takebayashi, Yumiko ;

Kasahara, Hiroyuki ;

Kamiya, Yuji ;

Zhao, Yunde .

PLANT AND CELL PHYSIOLOGY, 2014, 55 (06) :1072-1079

[5] Auxin synthesized by the YUCCA flavin Monooxygenases is essential for embryogenesis and leaf formation in Arabidopsis [J].

Cheng, Youfa ;

Dai, Xinhua ;

Zhao, Yunde .

PLANT CELL, 2007, 19 (08) :2430-2439

[6] A role for auxin in flower development [J].

Cheng, Youfa ;

Zhao, Yunde .

JOURNAL OF INTEGRATIVE PLANT BIOLOGY, 2007, 49 (01) :99-104

[7] Auxin biosynthesis by the YUCCA flavin monooxygenases controls the formation of floral organs and vascular tissues in Arabidopsis [J].

Cheng, Youfa ;

Dai, Xinhua ;

Zhao, Yunde .

GENES & DEVELOPMENT, 2006, 20 (13) :1790-1799

[8]

Dobrev PI, 2002, J CHROMATOGR A, V950, P21, DOI 10.1016/S0021-9673(02)00024-9

[9] Purification and determination of plant hormones auxin and abscisic acid using solid phase extraction and two-dimensional high performance liquid chromatography [J].

Dobrev, PI ;

Havlícek, L ;

Vágner, M ;

Malbeck, J ;

Kamínek, M .

JOURNAL OF CHROMATOGRAPHY A, 2005, 1075 (1-2) :159-166

[10] Pfam: the protein families database [J].

Finn, Robert D. ;

Bateman, Alex ;

Clements, Jody ;

Coggill, Penelope ;

Eberhardt, Ruth Y. ;

Eddy, Sean R. ;

Heger, Andreas ;

Hetherington, Kirstie ;

Holm, Liisa ;

Mistry, Jaina ;

Sonnhammer, Erik L. L. ;

Tate, John ;

Punta, Marco .

NUCLEIC ACIDS RESEARCH, 2014, 42 (D1) :D222-D230

← 1 2 3 4 5 →