A polymeric molecular "Handle" for multiple AFM-based single-molecule force measurements

被引：31
|
作者：
Valle, Francesco [1 ,2 ]
Zuccheri, Giampaolo [1 ,2 ,5 ]
Bergia, Anna [1 ]
Ayres, Lee [6 ]
Rowan, Alan E. [4 ]
Nolte, Roeland J. M. [4 ]
Samori, Bruno [1 ,2 ,3 ]
机构：
[1] Univ Bologna, Dept Biochem, I-40126 Bologna, Italy
[2] Univ Bologna, Natl Inst Phys Matter S3, I-40126 Bologna, Italy
[3] Univ Bologna, Interdepartmental Ctr L Galvani, I-40126 Bologna, Italy
[4] Radboud Univ Nijmegen, Inst Mol & Mat, NL-6525 ED Nijmegen, Netherlands
[5] Univ Bologna, Italian Interuniv Consortium Mat Sci & Technol, I-40126 Bologna, Italy
[6] Encapson Co, NL-6503 CB Nijmegen, Netherlands
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 13期
关键词：
atomic force microscopy; molecular recognition; polymers; scanning probe microscopy; single-molecule studies;
D O I：
10.1002/anie.200704526
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
(Figure Presented) Get a grip! The strategy of single-molecule mechanical unfolding of multimodular proteins is extended to the investigation of any chemical bond by an appropriately tailored, comblike polymer bearing multiple instances of any desired chemical interaction or bond to be studied along its linear chain. This approach is used to study the Ni-(His)n-NTA complex (see picture: red, Ni-NTA; green, His tag; His = histidine; NTA = nitrilotriacetate). © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：2431 / 2434
页数：4
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