Polyclonal antibodies against native weak toxin Naja kaouthia discriminate native weak toxins and some other three-fingered toxins against their denaturated forms

被引:3
作者
Kryukova, E
Mordvintsev, DY
Daya, S
Utkin, YN
Tsetlin, VI
机构
[1] RAS, Shemyakin & Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia
[2] Rhodes Univ, Fac Pharm, ZA-6140 Grahamstown, South Africa
基金
俄罗斯基础研究基金会;
关键词
weak toxin; cobra Naja kaouthia; three-fingered toxins; polyclonal antibodies; ELISA;
D O I
10.1016/j.toxicon.2005.03.017
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Polyclonal antibodies obtained by immunization of rabbits with native form of weak toxin (WTX) from cobra Naja kaouthia venom efficiently interacted with WTX and a weak toxin from Naja oxiana venom, but not so with their denaturated forms. These antibodies could also bind with lower affinity other groups of three-fingered toxins: long-chain alpha-neurotoxins, muscarinic toxins and cytotoxins, but practically did not bind short-chain alpha-neurotoxins. The efficiency of toxin-antibody interaction depends on the group (weak toxins, long or short alpha-neurotoxins, cytotoxins etc.) to which the toxin belongs, but not on species of snake from which the toxin originates. There is a correlation between the results obtained and phylogenetic analysis of the three-fingered toxins which revealed that WTX is very close to other weak toxins, relatively close to long alpha-neurotoxins, cytotoxins and muscarinic toxins, but is distant from the short a-neurotoxins. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:24 / 30
页数:7
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