Immortalization of human esophageal keratinocytes by E6 and E7 of human papillomavirus type 16

被引：2

作者：

Sashiyama, H

Shino, Y

Kawamata, Y

Tomita, Y

Ogawa, N

Shimada, H

Kobayashi, S

Asano, T

Ochiai, T

Shirasawa, H

机构：

[1] Chiba Univ, Sch Med, Dept Mol Virol, Chuo Ku, Chiba 2608670, Japan

[2] Chiba Univ, Sch Med, Dept Surg 2, Chiba 2608670, Japan

来源：

INTERNATIONAL JOURNAL OF ONCOLOGY | 2001年 / 19卷 / 01期

关键词：

immortalization; HPV type 16; E6; E7; human esophageal keratinocytes;

D O I：

暂无

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Transduction of human papillomavirus type 16 (HPV16) E6/E7 into primary culture of human esophageal keratinocytes using a recombinant adenovirus prolonged the life-span, while untreated cells senesced within 14-16 population doublings (PDLs). Up-regulation of telomerase activity and acquisition of serum-resistant growth were observed in the esophageal keratinocytes with extended lifespan between 50 and 100 PDLs, and drastically increased after 100 PDLs. A keratinocyte sample with a polymorphism of Pro/Pro at codon 72 of p53 showed resistance to HPV16 E6/E7-induced life-span-extension and immortalization, in contrast to others with p53 polymorphisms of Arg/Arg or Arg/Pro, which did not. The high efficiency of E6/E7-induction by adenovirus vector also revealed the M1 and M2 stages of keratinocyte immortalization first described in this report.

引用

页码：97 / 103

页数：7

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