Profiling of Enzymatic Activities Using Peptide Arrays

被引：5

作者：

Thiele, Alexandra ^{[2
]}

Poesel, Stephanie ^{[3
]}

Spinka, Michael ^{[1
]}

Zerweck, Johannes ^{[4
]}

Reimer, Ulf ^{[4
]}

Reineke, Ulrich ^{[5
]}

Schutkowski, Mike ^{[1
]}

机构：

[1] Univ Halle Wittenberg, Inst Biochem & Biotechnol, Dept Enzymol, D-06120 Halle, Saale, Germany

[2] Univ Halle Wittenberg, Inst Agr & Nutr Sci, D-06120 Halle, Saale, Germany

[3] Max Planck Res Unit Enzymol Prot Folding, D-06120 Halle, Germany

[4] JPT Peptide Technol GmbH, D-12489 Berlin, Germany

[5] 3B Pharmaceut GmbH, D-12489 Berlin, Germany

来源：

MINI-REVIEWS IN ORGANIC CHEMISTRY | 2011年 / 8卷 / 02期

关键词：

Kinase; peptide microarray; phosphatase; protease; SPOT synthesis; substrate specificity; DEPENDENT PROTEIN-KINASE; SITE-SPECIFIC IMMOBILIZATION; SUBSTRATE-SPECIFICITY; SPOT-SYNTHESIS; ELECTROCHEMICAL DETECTION; PHOSPHORYLATION SITES; CHEMICAL-SYNTHESIS; INHIBITION ASSAY; NUCLEIC-ACID; MICROARRAYS;

D O I：

10.2174/157019311795177772

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Applications of peptide arrays in the field of enzyme profiling are described in detail. Focus is on assay principles for measuring activities of kinases, phosphatases or proteases and on substrate identification/optimization for kinases. Additionally, several examples for reliable identification of substrates for other enzymes like lysine methyl transferases and ATP-ribosyltransferases are given. Finally, use of high-density peptide microarrays for the simultaneous profiling of kinase activities in complex biological fluids like cell lysates or lysates of complete organisms will be analysed. All published examples of peptide arrays used for enzyme profiling are summarized comprehensively.

引用

页码：147 / 156

页数：10

共 146 条

[1]

ADLER G, 1999, P INT S INN PERSP SO, P221

[2]

AKITA S, 2008, BIOORG MED CHEM

[3] A semisynthetic epitope for kinase substrates [J].

Allen, Jasmina J. ;

Li, Manqing ;

Brinkworth, Craig S. ;

Paulson, Jennifer L. ;

Wang, Dan ;

Hubner, Anette ;

Chou, Wen-Hai ;

Davis, Roger J. ;

Burlingame, Alma L. ;

Messing, Robert O. ;

Katayama, Carol D. ;

Hedrick, Stephen M. ;

Shokat, Kevan M. .

NATURE METHODS, 2007, 4 (06) :511-516

[4]

Antohe Bogdan V., 2007, V381, P299

[5] A novel glass slide-based peptide array support with high functionality resisting non-specific protein adsorption [J].

Beyer, M ;

Felgenhauer, T ;

Bischoff, FR ;

Breitling, F ;

Stadler, V .

BIOMATERIALS, 2006, 27 (18) :3505-3514

[6] Combinatorial synthesis of peptide arrays onto a microchip [J].

Beyer, Mario ;

Nesterov, Alexander ;

Block, Ines ;

Koenig, Kai ;

Felgenhauer, Thomas ;

Fernandez, Simon ;

Leibe, Klaus ;

Torralba, Gloria ;

Hausmann, Michael ;

Trunk, Ulrich ;

Lindenstruth, Volker ;

Bischoff, F. Ralf ;

Stadler, Volker ;

Breitling, Frank .

SCIENCE, 2007, 318 (5858) :1888-1888

[7]

Beyer M, 2009, METHODS MOL BIOL, V570, P309, DOI 10.1007/978-1-60327-394-7_16

[8]

BODEM J, 2002, SPRING LAB MAN, P141

[9] A substrate peptide for the FLT3 receptor tyrosine kinase [J].

Boehmer, Frank-D. ;

Uecker, Andrea .

BRITISH JOURNAL OF HAEMATOLOGY, 2009, 144 (01) :127-130

[10] Identification of differentially activated cell-signaling networks associated with Pichinde virus pathogenesis by using systems kinomics [J].

Bowick, Gavin C. ;

Fennewald, Susan M. ;

Scott, Erin P. ;

Zhang, LiHong ;

Elsom, Barry L. ;

Aronson, Judith F. ;

Spratt, Heidi M. ;

Luxon, Bruce A. ;

Gorenstein, David G. ;

Herzog, Norbert K. .

JOURNAL OF VIROLOGY, 2007, 81 (04) :1923-1933

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