A novel gene that is up-regulated during recovery from cold shock in Drosophila melanogaster

被引：61

作者：

Goto, SG ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Environm Earth Sci, Sapporo, Hokkaido 0600810, Japan

来源：

GENE | 2001年 / 270卷 / 1-2期

基金：

日本学术振兴会;

关键词：

Frost; proline-rich region; signal peptide;

D O I：

10.1016/S0378-1119(01)00465-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Gene expression during recovery at 25 degreesC (rearing temperature) after cold shock (0 degreesC) was studied in Drosophila melanogaster using a subtraction technique. A novel gene (Frost, abbreviated as Fst) was considerably up-regulated during recovery after cold shock. In addition, a prolongation of cold shock was more effective for induction. In contrast to cold shock, Fst gene did not respond to heat shock. This gene is apparently the same as the unidentified gene, CG9434. Fst has high internal repeats not only in nucleotide but also in amino acid sequences. In addition, FST protein has a proline-rich region. The deduced amino acid sequence revealed a modular structure; i.e., a signal peptide in the N-terminal region followed by a long hydrophilic region. Therefore, this protein is likely to be directed into ER and secreted into extracellular space. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：259 / 264

页数：6

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