High prevalence of Gammapapillomaviruses (Gamma-PVs) in pre-malignant cutaneous lesions of immunocompetent individuals using a new broad-spectrum primer system, and identification of HPV210, a novel Gamma-PV type

被引：15

作者：

Bolatti, Elisa M. ^{[1
,2
]}

Hosnjak, Lea ^{[3
]}

Chouhy, Diego ^{[1
,2
]}

Re-Louhau, Maria F. ^{[1
]}

Casal, Pablo E. ^{[2
]}

Bottai, Hebe ^{[4
]}

Kocjan, Bostjan J. ^{[3
]}

Stella, Emma J. ^{[1
]}

Gorosito, Mario D. ^{[5
]}

Sanchez, Adriana ^{[6
]}

Fernandez Bussy, Ramon ^{[6
]}

Poljak, Mario ^{[3
]}

Giri, Adriana A. ^{[1
,2
]}

机构：

[1] Consejo Nacl Invest Cient & Tecn, Inst Biol Mol & Celular Rosario, Grp Virol Humana, Suipacha 590, RA-2000 Rosario, Santa Fe, Argentina

[2] Univ Nacl Rosario, Fac Ciencias Bioquim & Farmaceut, Area Virol, RA-2000 Rosario, Santa Fe, Argentina

[3] Univ Ljubljana, Fac Med, Inst Microbiol & Immunol, Zaloska 4, Ljubljana 1000, Slovenia

[4] Univ Nacl Rosario, Fac Ciencias Bioquim & Farmaceut, Area Estadist & Procesamiento Datos, Suipacha 531, RA-2000 Rosario, Santa Fe, Argentina

[5] Univ Nacl Rosario, Fac Ciencias Med, Div Anat Patol, RA-3100 Rosario, Santa Fe, Argentina

[6] Univ Nacl Rosario, Fac Ciencias Med, Div Dermatol, RA-3100 Rosario, Santa Fe, Argentina

来源：

VIROLOGY | 2018年 / 525卷

关键词：

Gammapapillomavirus; Gamma-PV PCR; CUT PCR assay; Actinic keratosis; HPV210; Genomic characterization; HUMAN-PAPILLOMAVIRUS TYPES; SQUAMOUS-CELL CARCINOMA; ACTINIC KERATOSES; HEALTHY SKIN; INFECTION; BETA; PCR; DNA; DIVERSITY; PROTEIN;

D O I：

10.1016/j.virol.2018.09.006

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Genus Garnrnapapillomavirus (Gamma-PV) is the most diverse and largest Glade within the Papillomaviridae family. A novel set of degenerate primers targeting the El gene was designed and further used in combination with the well-known CUT PCR assay to assess HPV prevalence and genus distribution in a variety of cutaneous samples from 448 immunocompetent individuals. General HPV, Gamma-PV and mixed infections prevalence were significantly higher in actinic keratosis with respect to benign and malignant neoplasms, respectively (p = 0.0047, p = 0.0172, p = 0.00001). Gamma-PVs were significantly more common in actinic keratosis biopsies than Beta- and Alpha-PVs (p = 0.002). The full-length genome sequence of a novel putative Gamma-PV type was amplified by 'hanging droplet' long-range PCR and cloned. The novel virus, designated HPV210, clustered within species Gamma-12. This study provides an additional tool enabling detection of HPV infections in skin and adds new insights about possible early roles of Gamma-PVs in the development of cutaneous malignant lesions.

引用

页码：182 / 191

页数：10

共 65 条

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