The content of astaxanthin, including its mono- and diesters, and photosynthetic pigments, has been analyzed in cells of the H. pluvialis strain IBCE-H17 under the combined, prolonged action of several inducers of astaxanthin accumulation. The effective induction of the astaxanthin synthesis, mainly in the form of monoesters of fatty acids, was shown after 20 days of cultivation under high-intensity light and with the addition of 1-2 g/L of sodium acetate to the culture medium. A simultaneous decrease in the content of chlorophylls and lutein in H. pluvialis cells was observed under these conditions. The use of sodium acetate in combination with sodium chloride did not lead to noticeable changes in the content of astaxanthin as compared to the use of sodium acetate alone. The obtained data can be helpful in the biotechnological production of Haematococcus biomass enriched with economically valuable compounds.
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Univ Tokyo, Dept Life Sci, Grad Sch Arts & Sci, Meguro Ku, 3-8-1 Komaba, Tokyo 1538902, Japan
Tokyo Univ Sci, Grad Sch Chem Sci & Technol, Shinjuku Ku, 12-1 Ichigaya Funagawara Machi, Tokyo 1620826, JapanUniv Tokyo, Dept Life Sci, Grad Sch Arts & Sci, Meguro Ku, 3-8-1 Komaba, Tokyo 1538902, Japan
Shimada, Naoya
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Iijima, Kazutoshi
Hashizume, Mineo
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Tokyo Univ Sci, Grad Sch Chem Sci & Technol, Shinjuku Ku, 12-1 Ichigaya Funagawara Machi, Tokyo 1620826, JapanUniv Tokyo, Dept Life Sci, Grad Sch Arts & Sci, Meguro Ku, 3-8-1 Komaba, Tokyo 1538902, Japan
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Ist Italiano Tecnol, Ctr Nano Sci & Technol PoliMi, Via Pascoli 70-3, I-20133 Milan, ItalyUniv Verona, Dipartimento Biotecnol, Str Le Grazie 15, I-37134 Verona, Italy
Alcocer, Marcelo J. P.
Bargigia, Ilaria
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Ist Italiano Tecnol, Ctr Nano Sci & Technol PoliMi, Via Pascoli 70-3, I-20133 Milan, ItalyUniv Verona, Dipartimento Biotecnol, Str Le Grazie 15, I-37134 Verona, Italy
Bargigia, Ilaria
Perozeni, Federico
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Univ Verona, Dipartimento Biotecnol, Str Le Grazie 15, I-37134 Verona, ItalyUniv Verona, Dipartimento Biotecnol, Str Le Grazie 15, I-37134 Verona, Italy
Perozeni, Federico
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Cazzaniga, Stefano
Cerullo, Giulio
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Politecn Milan, Dept Phys, CNR, IFN, Pza L da Vinci 32, I-20133 Milan, ItalyUniv Verona, Dipartimento Biotecnol, Str Le Grazie 15, I-37134 Verona, Italy
Cerullo, Giulio
D'Andrea, Cosimo
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Ist Italiano Tecnol, Ctr Nano Sci & Technol PoliMi, Via Pascoli 70-3, I-20133 Milan, Italy
Politecn Milan, Dept Phys, CNR, IFN, Pza L da Vinci 32, I-20133 Milan, ItalyUniv Verona, Dipartimento Biotecnol, Str Le Grazie 15, I-37134 Verona, Italy