Standardization of a rapid spectrophotometric method for lycopene analysis

被引：39

作者：

Anthon, Gordon ^{[1
]}

Barrett, Diane M. ^{[1
]}

机构：

[1] Univ Calif Davis, Dept Food Sci & Technol, Davis, CA 95616 USA

来源：

PROCEEDINGS OF THE XTH INTERNATIONAL SYMPOSIUM ON THE PROCESSING TOMATO | 2007年 / 758期

关键词：

tomato; lycopene; analysis; spectrophotometric;

D O I：

10.17660/ActaHortic.2007.758.12

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Health professionals and consumers are becoming increasingly aware of the benefits of lycopene consumption; tomatoes provide the greatest source of this important phytonutrient. Our laboratory has developed a standard method for the extraction and determination of lycopene in tomato products. This is a sensitive, rapid and reproducible assay utilizing a mixture of hexane/ethanol/acetone for extraction. Following incubation for 1 hour, the hexane layer is removed using a Drummond pipettor and lycopene is determined by measuring absorbance at 503 nm. This method will be useful for breeding programs and processors who are interested in evaluation of a large number of samples in a rapid fashion. In this manuscript we describe the method and how it was validated.

引用

页码：111 / 127

页数：17

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